Methods Recombinant wild-type (WT) and variant MK proteins N-terminally fused to a maltose binding protein (MBP) were expressed in E. coli. Following affinity purification and size exclusion chromatography, the MBP-MK fusion (fMK) or cleaved MK (cMK) proteins, respectively, were characterized with regard to oligomerization, thermal unfolding monitored by differential scanning fluorimetry, therm...

Drug counterfeiting has exaggerated dramatically over the past decade causing a significant threat to human health. It must be processed in regard to medicines and the matter isn't restricted to a violation of belongings rights, however clearly involves every kind of poor quality pharmaceutical merchandise, including falsified and substandard medicine. The present paper discuss the review of th...

The direct determination in drinking water of perylene, chrysene, pyrene, benzo[a]pyrene, and benzo[k]fluoranthene, by front-face synchronous fluorimetry on a commercial SPE disk, has been evaluated. Sorbent treatment, influence of humic substances, and pH effect are discussed. In pure water the detection limits were estimated to be in the range 0.03-0.01 microg L(-1). A working pH in the range...

A high performance liquid chromatography has been adapted to the measurement of putrescine, spermidine and spermine in plasma and erythrocytes. Detection was by fluorimetry using o-phthalaldehyde which formed fluorophores with the polyamines. Sensitivity of the method was better than 10 pmol of each polyamine, and recovery averaged 93.9 percent. The precision of the analysis was two percent (R....

The nanoDSF (minituarized differential scanning fluorimetry) technology is a powerful method to determine the thermal and chemical stability of proteins by following changes in fluorescence, where the amino acid tryptophan serves as the main source for the fluorescence signal. We analyzed the stability of the light-oxygenvoltage (LOV) protein of the marine phototrophic α-proteobacterium Dinoros...

An improved, specific and sensitive method for the determination of the coproporphyrin isomers I and III is described. In this method, the hydrolysis of the coproporphyrin methyl esters takes place in the silica gel layer of acid-resistant pre-coated thin layer plates. After chromatography in the solvent system 2,6-dimethylpyridine/water, (volume ratio 20 ml + 6 ml), in an ammonia atmosphere, t...

Belton, N. R., Crombie, J. D., Robins, S. P., Stephen, R., and Farquhar, J. W. (1973). Archives of Disease in Childhood, 48, 472. Measurement of phenylalanine in routine care of phenylketonuric children. Fluorimetry, paper and column chromatography, and bacterial inhibition assay have been compared in the quantitation of serum or blood phenylalanine at levels ranging from 1 to over 30 mg/100 ml...

Damage of the normal tissue is a serious concenrn in cancer treatment. Hyperthermia by laserhas been considered as a safe cancer treatments methods with lower harmful effects on normaltissues. Using nanoparticles in cancer treatment has improved laser therapy, which is based ona selective cell targeting method to localize cell damages. Metallic nanoparticles such as gold,silver, and copper have...

Background: Laser-tissue interaction on low-level laser therapy (LLLT) has widespread medical applications (e.g., improved wound healing). The tensile strength of radiated tissue by LLLT is known to be increased mainly because of cross collagen bands developed after radiation.Objective: In this work, we studied the instantaneous effect of radiation of polarized laser beam on the viscoelastic ti...