Lipid nanoparticles (LNPs) containing short-interfering RNA (LNP-siRNA systems) are a promising approach for silencing disease-causing genes in hepatocytes following intravenous administration. LNP-siRNA systems generated by rapid mixing of lipids ethanol with siRNA aqueous buffer (pH 4.0) where the ionizable lipid is positively charged, followed dialysis to remove and raise pH 7.4. Ionizable c...

This work reports for the first time the detailed impacts of dual nitrogen (N) and phosphorus (P) stress on growth dynamics and biochemical composition in the Eustigmatophyte Nannochloropsis sp. P-stress concurrent with N-stress had subtle effects on culture bulk biochemical composition, but negatively influenced biomass productivity. However, the N:P supply ratio can be raised to at least 32:1...

Many green microalgae significantly increased their cellular neutral lipid content when cultured in nitrogen limited or high light conditions. Due to their lipid production potential, these algae have been suggested as promising feedstocks for biofuel production. However, no models for algal lipid synthesis with respect to nutrient and light have been developed to predict lipid production and t...

Response surface methodology (RSM) was used to enhance the biomass and lipid content in Nannochloropsis salina due to its economic importance. Preliminary screening results revealed that the heterotrophically cultivated N. salina with various carbon and nitrogen sources yielded higher biomass (0.91 ± 0.0035 g/L) and lipid content (37.1 ± 0.49 mg/L) than that of the photoautotrophical cultivatio...

Human T and B lymphocytes differ morphologically and immunologically although little is known concerning their physiological parameters and biochemical constituents. In this study, microviscosity and lipid composition in normal human B and T lymphocytes were investigated. Microviscosity was examined using 1,6-diphenyl-1,3,5-hexatriene as a probe and lipid composition was analyzed by thin-layer ...

because the ingestion of some types of dietary fibers has been shown to influence on the lipid and lipoprotein levels, it is possible that chitosan influences on lipid metabolism. in the present study, the effects of chitosan on the serum, liver lipid and lipoprotein concentrations in rat were investigated. serum lipid level in the treatment groups were decreased compared to that of the control...

Sprague-Dawley rats were fed for 4 months on a control diet or a polyunsaturated-fatty-acid (PUFA)-deficient diet. The combined effects of iron overload (Fe dextran) or Fe deficiency (desferrioxamine) on carrageenan-induced granuloma were studied. PUFA deficiency induced changes in Fe metabolism, but no alterations in lipid peroxidation variables were observed. Inflammation implied an increase ...

Cholesterol plays a crucial role in modulating the physicochemical properties of membranes, thus influencing membrane transport drugs. In this paper, effects caused by cholesterol on chlorzoxazone (CZX), centrally acting muscle relaxant drug, were probed through molecular dynamics simulations. POPC was selected as model lipid, and three different concentrations (0%, 20%, 50% CHOL) considered. T...