DNA helicases are essential to many cellular processes including recombination, replication and transcription, and some helicases function in multiple processes. The helicases encoded by the Xeroderma pigmentosum (XP) B and D genes function in both nucleotide excision repair and transcription initiation. Mutations that affect the repair function of these proteins result in XP while mutations af...

We report a procedure for the large-scale purification of the Escherichia coli Rep protein, a helicase that is involved in the replication of the E. coli chromosome as well as a number of single-stranded bacteriophages. The procedure starts with E. coli cells harboring an overproducing plasmid, pRepO, in which the E. coli rep gene is under transcriptional control of the inducible lambda PL prom...

We describe a fluorescence assay that can be used to monitor helicase-catalyzed unwinding of duplex DNA continuously in real time. The assay is based on the observation that fluorescence resonance energy transfer (FRET) occurs between donor (fluorescein) and acceptor (hexachlorofluorescein) fluorophores that are in close proximity due to their covalent attachment to the 3' and 5' ends of the co...

RACE NAME PARTY CANVASS VOTES PERCENT U. S. Representative District 1 Louie Gohmert(I) REP 129,398 89.72% Charles F. Parkes, III LIB 14,811 10.27% ----------Race Total 144,209 ---------------------------------------U. S. Representative District 2 Ted Poe(I) REP 130,020 88.61% David W. Smith LIB 16,711 11.38% ----------Race Total 146,731 ---------------------------------------U. S. Representativ...

It is well recognized that security is vital for reliable operation of vehicular ad-hoc networks (VANETs). Location privacy is one of the main security challenges in VANETs, which is concerned with preventing an attacker from tracking a specific vehicle. In this paper, we propose a novel location privacy preservation scheme for VANETs using random encryption periods (REP). REP is based on a pri...

The MIT Faculty has made this article openly available. Please share how this access benefits you. Your story matters. The subject of representation theory in complex rank goes back to the papers [DM, De1]. Namely, these papers introduce Karoubian tensor categories Rep(GL t) ([DM, De1]), Rep(O t), Rep(Sp 2t), t ∈ C ([De1]), which are interpolations of the tensor categories of algebraic represen...

conclusions polymerase chain reaction has specificity and accuracy for identification of the organism and is able to differentiate biotypes. enterobacterial repetitive intergenic consensus sequence is one of the informative and discriminative methods for the analysis of v. cholerae diversity. the rep-pcr is a less informative and discriminative method compared to other methods for the analysis ...

The Escherichia coli Rep protein is a 3' to 5' SF1 DNA helicase required for replication of bacteriophage phiX174 in E. coli, and is structurally homologous to the E. coli UvrD helicase and the Bacillus stearothermophilus PcrA helicase. Previous crystallographic studies of Rep protein bound to single-stranded DNA revealed that it can undergo a large conformational change consisting of an approx...

Cotton leaf curl Burewala virus (CLCuBuV), belonging to the genus Begomovirus, possesses single-stranded monopartite DNA genome. The bidirectional promoters representing Rep and coat protein (CP) genes of CLCuBuV were characterized and their efficacy was assayed. Rep and CP promoters of CLCuBuV and 35S promoter of Cauliflower mosaic virus (CaMV) were fused with β-glucuronidase (GUS) and green f...