Polyamines naturally occur in all living beings and play an important role in the regulation of cell proliferation, differentiation, and functional stimulation of terminally differentiated cells. Our studies, using specific inhibitors of polyamine biosynthesis such as alpha-difluoromethylornithine and methylglyoxal-bis[guanylhydrazone] to prevent polyamine accumulation, have indicated that poly...

Thiamine deficiency leads to a well-defined biochemical lesion of carbohydrate metabolism with increase in pyruvate and lactate in the blood (Peters, 1936). Since Lohmann & Schuster (1937) showed that thiamine in the ester form of pyrophosphate is the coenzyme for the decarboxylation of pyruvic acid, it has been found to participate in several important reactions of carbohydrate metabolism, in ...

Treatment of Ehrlich-ascites-carcinoma-bearing mice with methylglyoxal bis(guanylhydrazone) alone or in combination with 2-difluoromethylornithine greatly enhanced the transfer of intragastrically administered radioactive putrescine and cadaverine into the carcinoma cells. Difluoromethylornithine alone did not have any effect on the accumulation of intestine-derived diamines in the tumour cells...

Methylglyoxal (MGO) is recognized as being the bioactive component responsible for antibacterial activity of m?nuka honey. MGO content was investigated by high-performance liquid chromatography (HPLC-UV), in isocratic elution, to assess occurrence this compound mono- and multi-floral honey samples representative different botanical geographic origins Italy. Specifically, 110 from sweet cherry t...

Salinity and drought stress are significant environmental threats, alone or in combination. The current study was conducted to investigate the morpho-physiology, osmotic adjustment, oxidative stress, antioxidant defense methylglyoxal detoxification of three rice genotypes from indica (cv. BRRI dhan29 dhan48) japonica Koshihikari) groups. Eighteen-day-old seedlings these were exposed either sali...

Glyoxal oxidase (GLOX) is an extracellular H2O2-generating enzyme produced by ligninolytic cultures of Phanerochaete chrysosporium. The production, purification, and partial characterization of GLOX from agitated cultures are described here. High-oxygen levels are critical for GLOX production as for lignin peroxidase. GLOX purified by anion-exchange chromatography appears homogeneous by NaDod-S...