Microdevices enable clinical diagnostics to be miniaturized for use at the point-of-care (POC). Microdevices can be composed of microfilters, microchannels, microarrays, micropumps, microvalves, and microelectronics, and these mechanical and electrical components can be integrated onto chips to analyze and control biological objects at the microscale. The miniaturization of diagnostic tests off...

background: the microarray technology is in needed of cost-effective, low background noise and stable substrates for successful hybridization and analysis. methods: in this research, we developed a three-dimentional stable and mechanically reliable microarray substrates by coating of two polymeric layers on standard microscope glass slides. for fabrication of these substrates, a thin film of ox...

High-throughput "omics" technologies bring new opportunities for biological and biomedical researchers to ask complex questions and gain new scientific insights. However, the voluminous, complex, and context-dependent data being maintained in heterogeneous and distributed environments plus the lack of well-defined data standard and standardized nomenclature imposes a major challenge which requi...

Reconstructing gene regulatory networks, especially the dynamic gene networks that reveal the temporal program of gene expression from microarray expression data, is essential in systems biology. To overcome the challenges posed by the noisy and under-sampled microarray data, developing data fusion methods to integrate legacy biological knowledge for gene network reconstruction is a promising d...

Abstract Cell-to-cell communication can be inferred from ligand–receptor expression in cell transcriptomic datasets. However, important challenges remain: global integration of cell-to-cell communication; biological interpretation; and application to individual population profiles. We develop ICELLNET, a transcriptomic-based framework integrating: 1) an original expert-curated database interact...

We report the development of a peptide microarray based on previously determined phosphorylation sites in chloroplast proteins. Altogether, 905 peptides were spotted as 15mers in nine replicates onto glass slides. We used the microarray for in vitro phosphorylation experiments and specifically assessed the peptide substrate spectrum of chloroplast casein kinase II (pCKII). To this end, native p...

DNA microarray technology is a new and powerful tool that allows the simultaneous analysis of a large number of nucleic acid hybridization experiments in a rapid and efficient fashion. The development of the DNA microarray chip has been driven by modern techniques of microelectronic fabrication, miniaturization and integration to produce what is referred to as "laboratory-on-chip" devices. The ...