Metabolism and differentiation of cultured cells are influenced by changes in cellular morphology. In this study, we investigated the differences cell proliferation neuronal NT2 monolayer (2D) spheroid (3D) cultures. culture, adhered extended on a tissue culture plate. For fabricated microwell chip comprising 195 circular microwells (600 ìm diameter) cutture plate, surface was modified with pol...

Herpes simplex virus types 1 (HSV-1) and 2 (HSV-2) are members of the Herpesviridae family. About 40% to 80% of the world populations are infected with HSV and its prevalence is high in Iran. The high prevalence of this virus in the community and the ability of the virus in causing fatal diseases among immunocompromised patients, have encouraged studies to be performed on HSV and suitable cell ...

A model of organ culture of 18 day old fetal rat intestine (Quaroni, 1985) was modified and characterized in the present work with the purpose of developing an in vitro model for the study of intestinal epithelial cell behaviour. Fragments of this intestine were kept in suspension culture for 7 days and then explanted onto collagen (type I) matrix. Within a day, the fragments became anchored to...

background: based on recent studies, there are controversial reports on the ca-pacity of tissue cyst forming of toxoplasma gondii rh strain. in this study, the capac-ity was evaluated by in vivo and in vitro experiments. methods : rh strain was subcutaneously inoculated to ten wistar rats. after one month, their blood, brain, tongue and diaphragm were collected and evaluated by mat, pcr, pathol...

objective: this study was performed to determine whether melatonin at physiological concentrations (0.01-10nm) could affect the proliferation and osteogenic differentiation of rat adscs in vitro. materials and methods: adscs were isolated from the fat of adult rats. after cell expansion in culture media and through three passages, osteogenesis was induced on a monolayer culture with osteogenic ...

Three dimensional multicellular aggregate, also referred to as cell spheroid or microtissue, is an indispensable tool for in vitro evaluating antitumor activity and drug efficacy. Compared with classical cellular monolayer, multicellular tumor spheroid (MCTS) offers a more rational platform to predict in vivo drug efficacy and toxicity. Nevertheless, traditional processing methods such as plast...