Oncogenicity tests have revealed that a nonmutated erbB oncogene induces renal adenocarcinoma in addition to erythroblastosis. The erbB oncogene is a truncated form of the chicken epidermal growth factor receptor that lacks the extracellular ligand-binding domain. Previously, the nonmutated erbB oncogene has been reported to cause only erythroblastosis. The expansion of the disease potential of...

Introduction Marek’s disease, an economically-important lymphoid neoplasm of chickens, is caused by oncogenic strains of Marek’s disease herpesvirus (MDV). The virus replicates in lymphocytes and the epithelia of the skin and feather tissues (1). Despite very successful vaccination with attenuated virus strains, vaccine failures do occur as field viruses evolve towards greater virulence. In the...

............................................................................................................................ 3 Introduction ...................................................................................................................... 4 Protein tyrosine kinases ............................................................................................... 4 Discovery and...

A wide host range cosmid vector has been constructed by insertion of the lambda cos site into the plasmid pRK2501. This cosmid, which is maintained in Agrobacterium tumefaciens and is compatible with the Ti plasmid, has been used to make a clone bank of the A. tumefaciens pTiA6 plasmid. Several pTiA6 cosmids have been used to complement Tn5-induced Ti plasmid mutations. Five avirulent mutations...

The intestinal epithelium maintains homeostasis by a self-renewal process involving resident stem cells, including Lgr5(+) crypt-base columnar cells, but core mechanisms and their contributions to intestinal cancer are not fully defined. In this study, we examined a hypothesized role for KLF5, a zinc-finger transcription factor that is critical to maintain the integrity of embryonic and induced...

By exposing an established cell line of malignant mouse lymphocytic cells (L5178Y) to a culture environment low in calcium and magnesium, we have isolated and maintained in continuous culture a variant population with reduced growth requirements for the cations. The variant cells are larger, have enhanced aggregability, tend to form dispersed colonies in semisolid medium, and have increased onc...

An avian leukosis virus with a wide host range belonging to a new subgroup for chickens was isolated from meat-type chicken lines. The virus, of which HPRS-103 strain is the prototype, was of low oncogenicity in chickens but appeared to behave like an exogenous leukosis virus. Neutralizing antibodies to the virus were found in three of five meat-type chicken lines, but not in seven layer lines....

Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiologic agent associated with Kaposi's sarcoma (KS), primary effusion lymphoma (PEL), and multicentric Castleman disease (MCD). Similar to other herpesviruses, KSHV has two life cycles, latency and lytic replication. In latency, the KSHV genome persists as a circular episome in the nucleus of the host cell and only a few viral genes are ex...

Introduction The Simian Virus 40 (SV40) large T antigen is multifunctional protein with DNA helicase, RNA helicase and ATPse activities which contribute to multistep tumorogenesis in rodents and humans. The Immortomouse mouse strain expresses a mutated large T antigen tsA58 oncogene under the control of the interferon inducible murine H-2K promoter on chromosome 16. Our aim was to establish a B...