We describe a new, simple immobilization protocol designed to induce muscle atrophy in lower hindlimb muscles in mice. Hindlimbs of C57BL6 mice were unilaterally immobilized for 7 days. Morphological analysis revealed that immobilization resulted in a significant reduction in muscle fiber size in both the extensor digitorum longus (EDL) and tibialis anterior (TA). By contrast, no significant ch...

Surface plasmon resonance (SPR) is a powerful label-free diagnostic tool to study biomolecular interactions. However, one of the drawbacks of SPR is the lack of controlled immobilization of ligands on the sensor surface. We have developed a modular platform for the fast, reagent-free and site-specific immobilization of azide-containing ligands by strain-promoted cycloaddition onto a cyclooctyne...

phospholipase a1 is known as an effective catalyst for hydrolysis of various phospholipids in enzymatic vegetable oil degumming. immobilization is one of the most efficient strategies to improve its activity, recovery and functional properties. in this study, chitosan-co-polyethylene oxide (90:10) nanofibrous mat was successfully fabricated and modified with atmospheric plasma at different time...

Many biotechnological applications use protein receptors immobilized on solid supports. Although, in solution, these receptors display homogeneous binding affinities and association/dissociation kinetics for their complementary ligand, they often display heterogeneous binding characteristics after immobilization. In this study, a fluorescence-based fiber-optic biosensor was used to quantify the...

Today, “Sustainable production” has attracted a great deal of interest by academic researchers and practitioners due to the raising environmental and social concerns. Sustainable EPQ model has been developed as a result of this interest and also necessity. This paper develops a novel sustainable EPQ (SEPQ) model under partial backordering consideration. The model converts all emission variation...

BACKGROUND Guidelines and clinical practice vary considerably with respect to thrombosis prophylaxis during plaster cast immobilization of the lower extremity. Identifying patients at high risk for the development of venous thromboembolism (VTE) would provide a basis for considering individual thromboprophylaxis use and planning treatment studies. The aims of this study were (1) to investigate ...

Brain-derived neurotrophic factor (BDNF) promotes survival and differentiation of neural cells in the central and peripheral nervous systems. BDNF has been detected in plasma, but its source has not yet been established. Expression of BDNF mRNA has been identified in the submandibular glands when male rats are exposed to acute immobilization stress. In the present study, we investigated whether...

A factor or factors released by cultured NG108-15 neuroblastoma X glioma hybrid cells and added to the medium of rat myotube primary cultures was found to immobilize some of the previously mobile acetylcholine receptors in the myotube membrane. Partial receptor immobilization occurred within 3 h after the beginning of treatment with the NG108-15-conditioned medium factor and persisted for at le...

+Christiansen, B A; Roberts, B J; Cloutier, A M; Manoharan, R K; Shah, S R; Bouxsein, M L; +Beth Israel Deaconess Medical Center, Boston, MA [email protected] INTRODUCTION: Mechanical loading is necessary for proper development and maintenance of the musculoskeletal system. There is a great deal of research in small animal models investigating the musculoskeletal response to increased ...