The crude polar extract of mangosteen fruit pericarp not only has a moderate antioxidant activity (55±4 μg/mL) but also high cytotoxicity (16±0.5 μg/mL). presumably is caused by the presence complex cytotoxic compounds from pericarp. To obtain non-toxic preparation with activity, 50% ethanol extracts were partially purified using reverse-phase column chromatography Silica C18 as stationary phas...

NADPH-cytochrome P450 (cytochrome c) reductase (EC 1.6.2.4) was solubilized by detergent from microsomal fraction of wounded Jerusalem-artichoke (Helianthus tuberosus L.) tubers and purified to electrophoretic homogeneity. The purification was achieved by two anion-exchange columns and by affinity chromatography on 2',5'-bisphosphoadenosine-Sepharose 4B. An Mr value of 82000 was obtained by SDS...

Staphylococcal hyaluronate lyase (hyaluronidase) derived from a pathogenic strain of staphylococcus was purified by means of salt fractionation with ammonium sulfate and gel filtration through Sephadex G-100. Most of the enzyme activity from concentrated culture supernatant fluids of staphylococci was obtained in a fraction precipitated by 90 to 100% saturation with ammonium sulfate. A small am...

the production of murine monoclonal antibody (mab) has not yet been reported in iran. the present work describes for the first time the generation of several murine hybridoma clones secreting mabs directed against human leukocyte surface antigens. the secreted antibodies by hybridoma clones have been screened on different lymphoid and non-lymphoid tissues. results indicated that of seven hybrid...

Cytidine-triphosphate synthetase (UTP: ammonia ligase (ADP-forming), EC 6.3.4.2) has been purified over 31,000-fold to homogeneity with 17% recovery from rat liver cytosol, using high-performance liquid chromatography (HPLC) techniques. The presence of CTP synthetase monomer, dimer and tetramer has been demonstrated in the ammonium sulfate fraction of rat liver cytosol. By gel-permeation HPLC, ...