background: serological diagnosis of toxoplasma gondii infection using crude antigens may not be more accurate. to increase the diagnostic potency of anti-gens, isolation of their immunogenic fractions could be useful. the current re-search adopted to obtain an affinity isolated fraction from rh strain using cnbr sepharose 4b column coupled with infected mice sera helping in detec-tion of igm a...

materials and methods total protein was determined by lowery method. bovine lens alr2 was partially purified by gel filtration chromatography on sephadextm g25. bovine cortex kidney alr1 was partially purified by diethylaminoethyl (deae) precipitation. the hydroalcoholic extract was obtained from frozen propolis. the enzyme activity and sorbitol accumulation in erythrocytes were determined spec...

Partially purified antigenic fractions of Leishmania major promastigotes were obtained by sodium dodecyl sulfate (SDS-PAGE) and electro-elution. Three isolated protein fractions designated as frac‌tions (Fr.) 1, 2, and 3 correspond to 40-60, 60-80, 80-100 kilodalton (kDa) respectively. They were used for immunization of BALB/c mice against Leishmaniasis. The effects of these fractions on immune...

partially purified antigenic fractions of leishmania major promastigotes were obtained by sodium dodecyl sulfate (sds-page) and electro-elution. three isolated protein fractions designated as frac tions (fr.) 1, 2, and 3 correspond to 40-60, 60-80, 80-100 kilodalton (kda) respectively. they were used for immunization of balb/c mice against leishmaniasis. the effects of these fractions on immune...

Antibodies directed against the enzyme glutamic acid decarboxylase (GAD) are believed to be the main cause of destruction of pancreatic islet cells in type I (insulin dependent) diabetes mellitus. The enzyme was found both in the brain and pancreatic beta cells. Although similarities in identity of GAD in human and rat brain have been demonstrated, little is known about the interaction betw...

Since, we previously demonstrated that sequentially extracted methanolic fraction showed marked antioxidant and antidiabetic property in vitro, the present study was design to evaluate the beneficial effects of Phyllanthus virgatus methanolic extract and its partially purified fraction on hyperglycemia and hyperlipidemia in streptozotocin (STZ) induced diabetic rats. The plant extract was subje...