BACKGROUND AND OBJECTIVES Natural killer (NK)-92 cells are effective against a broad range of malignant targets both in vitro and in vivo. Interleukin-15 (IL-15) is an important cytokine for NK cell development and differentiation. IL-15 gene-modified NK-92 cells need to be characterized and their clinical implications investigated. DESIGN AND METHODS IL-15 cDNA was inserted into a pcDNA3 euk...

We report a novel t(7;9)(q11;p13) translocation in 2 patients with B-cell acute lymphoblastic leukemia (B-ALL). By fluorescent in situ hybridization and 3' rapid amplification of cDNA ends, we showed that the paired box domain of PAX5 was fused with the elastin (ELN) gene. After cloning the full-length cDNA of the chimeric gene, confocal microscopy of transfected NIH3T3 cells and Burkitt lympho...

Nonobese diabetic (NOD) mice develop insulitis and diabetes through a process involving autoimmunity to the 60-kDa heat shock protein (HSP60). Treatment of NOD mice with HSP60 or with peptides derived from HSP60 inhibits this diabetogenic process. We now report that NOD diabetes can be inhibited by vaccination with a DNA construct encoding human HSP60, with the pcDNA3 empty vector, or with an o...

Gene regulation circuits control all aspects of the life of plasmids. This review gives an overview of the current orchestration of the circuits that control plasmid replication, plasmid transfer, plasmid segregation and plasmid maintenance.

background: p19 cells are mouse embryonic carcinoma cells which contain pluripotent ability, like stem cells, to differentiate into different cell lines. there are several properties for this cell line that make it a valuable cell model for study of developmental stages. methods: at the first step, pts2-egfp coding sequence which was cloned in pucd2.hygro vector was used for transfection in to ...

Background: The importance of plasmid-encoded Extended Spectrum Beta Lactamase (ESBL) organisms in the dissemination multi-drug resistance and complicated infections are contributory factors to treatment failure. Despite these, there is a paucity information regarding plasmid-borne associated infection from primary sources including cockroaches while studies correlating total protein ESBL also ...

ABSTRACT Plasmids are common in natural bacterial communities, facilitating evolution via horizontal gene transfer. Bacterial species vary their proficiency to host plasmids: whereas plasmids stably maintained some regardless of selection for plasmid-encoded genes, other species, even beneficial rapidly lost. It is, however, unclear how this variation affects plasmid persistence communities. He...

Some years ago, we found that Axial organ (AO) cells exerted an induced and spontaneous cytotoxicity against SP2 MBL2 mouse tumoral cells. Recently, discovered a sea star Igkappa gene with immune properties. This was first, inserted in CMV (cytomegalovirus) finally plasmid called « young » plasmid. In the present experiment IGKappa cloned HeK human to produce specific protein. The induced« prot...