the lipophilic yeasts of malassezia species are members of the normal skin microbial that are cause of pityriasis versicolor. pityriasis versicolor is a common superficial fungal infection with world-wide distribution. the phenotypic methods for identification of malassezia species usually are time consuming and unreliable to differentiate newly identified species. but dna-based techniques rapi...

q fever is a zoonotic disease that occurs worldwide and is caused by the obligate intracellular bacterium coxiella burnetii. the aim of this study was to investigate the presence of c. burnetii infection in aborted sheep in eastern turkey using pcr. a total of 200 fetuses were collected from aborted sheep belonging to 200 herds in different locations in the eastern part of turkey. foetal organ ...

STUDY OBJECTIVE To assess the value of Aspergillus polymerase chain reaction (PCR) test performed on the BAL in diagnosing invasive pulmonary aspergillosis (IPA). DESIGN Between January 1996 and 1997, we prospectively followed up 249 cancer patients with pulmonary infiltrates suggestive of pneumonia. Bronchoscopy with fungal stains, cultures, and PCR was performed on all patients. PCR was use...

introduction recently acinetobacter baumannii isolates have emerged as a problematic infectious agent that causes meningitis in neurosurgical patients. colistin has been used successfully for the treatment of a. baumannii meningitis but colistin resistant isolates have been reported worldwide. case presentation two isolates of a. baumannii were cultured during a five-day period from cerebrospin...

A real-time PCR was designed for detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae such that each pathogen could be detected in a single tube and differentiated using molecular beacons marked with different fluorochromes. This duplex PCR, targeting the P1 adhesion gene for M. pneumoniae and the ompA gene for C. pneumoniae, was compared with two conventional PCR assays targeting th...

Aminoglycoside-modifying enzymes (AMEs) and 16S rRNA methylases (16S RMTase) are two main resistance mechanisms against aminoglycosides. This study aimed to evaluate the frequency of AMEs methylase genes among aminoglycoside non-susceptible Acinetobacter baumannii isolates assess their clonal relationship using repetitive extragenic palindromic-PCR (rep-PCR). In this cross-sectional study, a to...