It is demonstrated that carboxypeptidase Y can be used to exchange the C-terminal alanyl residue of porcine insulin with a threonyl residue, thus forming human insulin. Using threonine amide as nucleophile, the reaction proceeds as a transpeptidation via human insulin amide, des(Ala)a30(Thr-NH2)a30insulin. However, since the amidase activity of carboxypeptidase Y towards this particular insulin...

The mode-specific vibrational energy relaxation of the amide I' and amide II' modes in NMA-d(1)/(D(2)O)(n) (n = 0-3) clusters were studied using the time-dependent perturbation theory at the B3LYP/aug-cc-pvdz level. The amide modes were identified for each cluster based on the potential energy distribution of each mode. The vibrational population relaxation time constants were derived for the a...

Delavirdine mesylate (U-90152T) is a highly specific nonnucleoside HIV-1 reverse transcriptase inhibitor currently under development for the treatment of AIDS. The excretion, disposition, brain penetration, and metabolism of delavirdine were investigated in CD-1 mice after oral administration of [14C]delavirdine mesylate at single doses of 10 and/or 250 mg/kg and multiple doses of 200 mg/kg/day...

The mechanism of the vasodilator effect of procaine amide in dogs was investigated in isolated gracilis muscle and hindpaw perfused at constant flows with arterial blood. Inhibition of sympathetic ganglionic transmission contributed predominantly to the vasodilatation in muscle that accompanied intravenous administration of procaine amide (20 mg/kg). In contrast, comparable amounts of procaine ...

Abnormal aggregation of amyloid-β is a very complex and heterogeneous process. Owing to methodological limitations, the pathway still not fully understood. Herein new approach presented in which secondary structure single aggregates investigated with tip-enhanced Raman spectroscopy (TERS) liquid environment. Clearly resolved TERS signatures amide I III bands enabled detailed analysis molecular ...

Infrared spectroscopy of biological cell monolayers grown on surfaces is a poorly developed field. This is unfortunate because these monolayers have potential as biological sensors. Here we have used infrared microscopy, in both transmission and transflection geometries, to study air-dried Vero cell monolayers. Using both methods allows one to distinguish sampling artefactual features from real...

In the title compound, C(14)H(11)Cl(2)NO, the two benzene rings are non-coplanar [dihedral angle = 60.9 (3)°]. In the crystal, an amide N-H⋯O hydrogen bond links the mol-ecules into chains which extend along (001).

In the title compound, C(20)H(24)N(2)O(5), the dihedral angle between the two roughly planar salicyl-amide fragments [r.m.s. deviations = 0.043 (2) and 0.149 (2) Å] is 25.50 (5)°. The mol-ecular conformation is stabilized by intra-molecular O-H⋯O hydrogen bonds involving phenol -OH groups and amide O atoms. Inter-molecular hy-droxy-meth-yl-amide O-H⋯O and amine-hy-droxy-methyl N-H⋯O hydrogen bo...

It was previously reported that a midregion domain of parathyroid hormone-related protein (PTHrP), that is, [67-86]-amide, is able to restrain growth and promote matrigel penetration by the 8701-BC cell line, derived from a biopsy fragment of a primary ductal infiltrating carcinoma of the human breast, and that cell invasion in vitro is drastically impaired by inactivation of urokinase-plasmino...