Background: A rapid microtiter plate based sandwich hybridization assay was developed for detection and quantification of single RNA species using magnetic beads. Following solution hybridization target RNA molecules were collected by biotin-streptavidin affinity binding and detected by fluorescence signal generated by alkaline phosphatase. The 18S rRNA and SUC2 mRNA of Saccharomyces cerevisiae...

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This paper studies the multi-factory production (MFP) network scheduling problem where a number of different individual factories join together to form a MFP network, in which these factories can operate more economically than operating individually. However, in such network which known as virtual production network with self-interested factories with transportation times, each individual facto...

There has been a growing trend toward the automated generation of massive data at multiple distributed locations, leading to a future of computing that is data-rich, heterogeneous, distributed, and rife with uncertainty. Examples include systems to monitor the physical world, such as wireless sensor networks, and systems to monitor complex infrastructures, such as distributed Internet monitors....

After the establishment of the Kobayashi-Maskawa mechanism of CP violation at the two B factories, possibilities to increase the integrated luminosity by two orders of magnitude have been investigated, since it seems to be the amount needed to find physics beyond the Standard Model through CP violating and other observables in rare B meson decays, D meson decays and τ lepton decays. This report...

Transgenic plants are promising alternatives for the production of recombinant pharmaceutical proteins (plant molecular farming). Plants as higher eukaryotes perform posttranslational modifications similar to those of mammalian cell lines. Low-cost cultivation and safe pathogenfree production are further advantages. However, field cultivation of transgenic plants raises social, environmental an...

Myxobacteria are amongst the top producers of natural products. The diversity and unique structural properties of their secondary metabolites is what make these social microbes highly attractive for drug discovery. Screening of products derived from these bacteria has revealed a puzzling amount of hits against infectious and non-infectious human diseases. Preying mainly on other bacteria and fu...

(El a-ABSTRACT We briefly review the physics of CP violation and the interest of studying this phenomenon in the B-meson system. The need for very large numbers of B-decays is shown, and a number of approaches for B-factories are compared. In particular, e+e-linear and circular colliders are discussed in some detail, with specific examples presented .

class Application { List docs; protected abstract new Document(); public void newDocument() { // Handles the File|New menu option doc = new Document(); docs.add(doc); doc.open(); } // ... rest of the class omitted } (a) The abstract Application class class MyApplication extends Application { protected new Document() { return new MyDocumentType(); // A concrete subtype } // ... rest of...