herpes simplex virus 1 (hsv-1) unspliced 8.3 latency associated transcript (lat), which located in the long repeat sequences, has been shown to contain at least 16 open reading frames (orf: a-p). one of these orf, orf p, maps almost entirely antisense to hsv-1 neurovirulence gene, icp34.5. both orf p and icp34.5 are located in the long repeat and are antisense overlapping genes. therefore, in o...

We have developed a pull-down, menu-based integrated environment for electronic data interchange according to the UN/EDIFACT standard. The software package runs under the MS-DOS operating system and supports all phases of an interchange, from designing an interchange agreement to the nal transmission of generated EDIFACT messages. This paper describes the solutions that introduce the open-EDI s...

We show in vitro synthesis of a fully functional protein that binds substrate with high affinity. After reconstitution into proteoliposomes, the in vitro synthesized protein actively transports substrate. Coexpression of two plasmids in the Rapid Translation System RTS allowed the demonstration of functional complementation. Pull-down experiments confirmed that the basic functional unit of EmrE...

This study investigates the performance of nanorefrigerants (R134a-ZrO2) in a domestic refrigerator at concentration 0.2 g/l without changing components. Nanoparticles ZrO2 g/L with particle size 1-10 nm and 140 g R134a have been charged, investigations were carried out. Energy consumption pull-down tests conducted to investigate refrigerator. The parameters like refrigeration capacity, compres...

Culture of entrepreneurs is also a sort of capital, which has a direct effect on development of enterprises in the process of economic operation of enterprises. Construction of cultural capital among entrepreneurs helps to enhance the overall competitive strength of Chinese enterprises in international society and helps to push forward development of the economic society. Due to historical reas...

A novel post-translational modification of threonine, β-N-acetylglucosaminyl-phosphate, was recently discovered on assembly protein AP180, a protein which plays a crucial role in clathrin coated vesicle formation in synaptic vesicle endocytosis (SVE). Herein, we report studies aimed at probing the effect of this modification on binding to proteins in rat brain lysate using pull down experiments...