Site-directed mutagenesis previously identified the residues responsible for the biological activity of the plant defense legume lectin, Griffonia simplicifolia lectin II (GSII) [Proc. Natl. Acad. Sci. USA 95, (1998) 15123-15128]. However, these results were inconclusive as to whether these residues function as direct defense determinants through carbohydrate binding, or whether substantial cha...

Abstract: Chemoselective ligation reactions are highly efficient and specific covalent bond forming reactions capable of proceeding within a physiological environment. Chemoselective ligations offer exquisite specificity, similar to enzymatic reactions, but with the significant advantage of accessing a much broader range of coupling partners. Thus, even among a multitude of reactive functional ...

Monoclonal mouse antibody to streptococcal group A carbohydrate was evaluated in diagnostic microbiology laboratory. A total of 262 isolates of beta-hemolytic streptococci were classified with commercial reagents and the monoclonal antibody to group A carbohydrate by using immunofluorescence and Lancefield precipitin tests. Both nonoclonal and commercial antibodies were identifically reactive b...

Using murine (T,G)-A--L-reactive T cell clones, we have demonstrated the existence of unique homozygous antigen-presenting determinants expressed on C57bl/6 mice, controlled by the I-A subregion of the murine major histocompatibility complex (MHC), which are not expressed on semisyngeneic (C57Bl/6 x A/J)F1 [(B6A)F1] cells. Additionally, we were able to demonstrate that there exist (T,G)-A--L-re...

Rabbit anti-idiots~ic antisera specific for anti-p-azophenylarsonate (antiAr) t antibodies, raised in an individual strain A / J mouse, recognize determinants on anti-Ar antibodies induced in all members of this strain so far tested. These determinants have been found only on anti-At antibodies and not on normal imnmnoglobulins or on other anti-hapten or anti-protein antibodies induced in this ...

Acute myeloid leukemia (AML) is a heterogeneous disease with poor clinical outcomes. We have previously shown that constitutive activation of NADPH oxidase 2 (NOX2), resulting in over-production reactive oxygen species (ROS), occurs over 60% AML patients. also increased ROS production promotes glucose uptake and proliferation cells, mediated by changes carbohydrate metabolism. Given carbohydrat...

We present evidence that a highly purified pepsin extract of type 5 streptococcal M protein (pep M5) contains at least three epitopes that are cross-reactive with sarcolemmal membrane proteins of human myocardium. The tissue-cross-reactive determinants of pep M5 are also partially shared with pep M6 and pep M19. Three rabbits immunized with a single 300 micrograms dose of pep M5 developed signi...

Pulmonary Hypertension is a progressive vascular disease resulting from the tapering of pulmonary arteries causing high arterial blood pressure and ultimately right ventricular failure. A defining characteristic excessive remodeling that includes increased proliferation, fibrosis inflammation. There no outward cure for nor are there interventions effectively impede or reverse remodeling, resear...