background: allergens are mostly composed of glycoprotein structures. it is believed that glycan-specific antibodies may lead to false-positive reactions in immunoassays. in this study we investigated the glycosylation state of grape allergens as well as the presence of antibodies to cross-reactive carbohydrate determinants (anti-ccds) in sera from grape-sensitive individuals. methods: grape ex...

A relevant proportion of allergy diagnosis is accomplished by in vitro determination of specific immunglobulin E (sIgE) to extracts from suspected allergens. Such extracts inevitably contain glycoproteins, which may react with patients' IgE. In the case of plant and insect allergens, the relevant epitope structure is an α-1,3-fucose on the Asn-linked sugar residue of so-called N-glycans. Due to...

background: several factors are associated with the development or exacerbation of pain in knee osteoarthritis (koa). in this study, we reviewed this context based on relevant studies. methods: recent published studies which have addressed the relationship between pain and koa were summarized. results: correlates of the clinical, demographic features, laboratory tests and abnormalities on radio...

performed to 16 foods and 38 inhalant allergens. Patch testing to foods was also performed. IgE-mediated allergy was diagnosed if either serum-specific IgE or skinprick test results were positive, whereas non–IgE-mediated allergy was diagnosed if a positive patch test result was found. A streptavidin-based immunoassay was performed to determine the presence of cross-reactive carbohydrate determ...

performed to 16 foods and 38 inhalant allergens. Patch testing to foods was also performed. IgE-mediated allergy was diagnosed if either serum-specific IgE or skinprick test results were positive, whereas non–IgE-mediated allergy was diagnosed if a positive patch test result was found. A streptavidin-based immunoassay was performed to determine the presence of cross-reactive carbohydrate determ...

performed to 16 foods and 38 inhalant allergens. Patch testing to foods was also performed. IgE-mediated allergy was diagnosed if either serum-specific IgE or skinprick test results were positive, whereas non–IgE-mediated allergy was diagnosed if a positive patch test result was found. A streptavidin-based immunoassay was performed to determine the presence of cross-reactive carbohydrate determ...