Bulk tank milk samples from 392 Northern Ireland dairy farms and individual animals (n = 293) on 4 of these were tested by a novel phagomagnetic separation (PhMS)-quantitative (q)PCR assay able to detect quantify viable Mycobacterium avium ssp. paratuberculosis (MAP), demonstrate its potential utility as surveillance tool. Viable MAP detected in 26.5% the bulk milks, with contamination levels r...

The bovine viral diarrhea virus (BVDV), a single-stranded RNA virus, can cause fatal diarrhea syndrome, respiratory problems, and reproductive disorders in herds. Over the past few years, it has become clear that the BVDV infection rates are increasing and it is likely that an effective vaccine for BVDV will be needed. In this study, transgenic Astragalus was used as an alternative productive p...

an outbreak of a lethal disease was reported in 4–6-month-old holstein calves in a feedlot around tehran. the signs of central nervous system and gastrointestinal system (gi) involvement were observed in the diseased animals. necropsy samples of gi, liver, kidney, spleen and lung from 3 died animals were prepared for histopathological examination. blood and formalin-fixed ear notch samples of 6...

An outbreak of a lethal disease was reported in 4–6-month-old Holstein calves in a feedlot around Tehran. The signs of central nervous system and gastrointestinal system (GI) involvement were observed in the diseased animals. Necropsy samples of GI, liver, kidney, spleen and lung from 3 died animals were prepared for histopathological examination. Blood and formalin-fixed ear notch samples of 6...

Twelve batches of commercially available foetal bovine sera were tested for the presence of bovine viral diarrhoea virus (BVDV) and BDVD antibodies. The antibodies were found in three batches coming from two suppliers. Fragments of BVDV genome were present in five of these batches when tested by RT-PCR. All of them were type 1 BVDV according to a direct sequencing of PCR products. However, the ...