Chloroplast microsatellite primers were developed in order to provide more population genetic information of endangered Rheum officinale, R. palmatum, and R. tanguticum for conservation. The dried roots and rhizomes of these plants are important in traditional Chinese medicine. The results showed that the optimum concentrations of Mg(2+), Taq DNA polymerase, dNTPs, template DNA, and primers in ...

ankylosing spondylitis and related spondyloarthropathies. Rheum Dis Clin North Am 1995;21:117–28. 2. D’Agostino MA, Breban M, Said-Nahal R, Dougados M. Refractory inflammatory heel pain in spondylarthropathy: a significant response to infliximab documented by ultrasound. Arthritis Rheum 2002;46:840–1. 3. Olivieri I, Padula A, Barozzi L. The enthesiopathy: clinical manifestations, imaging and tr...

We developed a new approach using RAPD fingerprints to distinguish 37 currant cultivars from northeastern China based on optimization of RAPD by choosing 11 nucleotide primers and strict screening PCR annealing temperature. We found that the manual cultivar identification diagram (MCID) approach clearly developed fingerprints from 8 different primers that were useful for cultivar identification...

Rheum palmatum, one of the source plants of the traditional Chinese medicine rhubarb, is anendemic and endangered species. To our knowledge, this is the first report on the chromosome number and karyotype of this species. Sectioning combined with micrography was used to analyze the karyotype. The following results were obtained: R. palmatum had a stable chromosome number 2n = 22; the basic numb...

Antimicrobial activity of Ribes nigrum L. and rubrum leaf extracts was determined by disk-diffusion method as well specific growth rate generation succeeding factor determination. Different Gram-positive (Bacillus subtilis WT-A, Staphylococcus aureus MDC 5233, Enterococcus hirae ATCC 9790) Gram-negative (Escherichia coli 25922, Salmonella typhimurium MDC1754, ampicillin resistant E. dhpα-pUC18 ...