deficiency. It is not applicable to newborn or mass screening with use of ifiterpaper specimens, but is satisfactory for liquid urine screening. In one variant of sulfite oxidase deficiency, due to a defect of its molybdate cofactor metabolism, the concentration of uric acid in serum is decreased. Thus, an unexplained low serum uric acid, especially in a young patient with neurologic abnormalit...

In this study, the synthesis of ceria (CeO2) nanoparticles (NPs) was examined by the biosynthesis method. Then, enzyme-like features of synthesized nanoceria were examined. Peroxidase enzyme from fig (Ficus carica) was used as a synthesis and stabilizer reagent. Furthermore, it was investigated whether the obtained nanoceria has superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX) ac...

Recombination systems based on lambda and Cre/loxP have been described to facilitate gene transfer from one vector to another in a high-throughput fashion, avoiding the bottlenecks associated with traditional cloning. However, no system described to date is suitable for the cloning of affinity reagents selected from display libraries, which requires that the recombination signals flanking the a...

We developed a new approach for chemical ligation of oligonucleotides using the electrophilic phosphorothioester (EPT) group. A nucleophilic phosphorothioate group on oligonucleotides was converted into the EPT group by treatment with Sanger's reagent (1-fluoro-2,4-dinitrobenzene). EPT oligonucleotides can be isolated, stored frozen, and used for the ligation reaction. The reaction of the EPT o...

THE POOR QUALITY of reagent chemicals in the United States by 1916 led to the statement by Hillebrand (1) that “the situation is particularly deplorable just now.” In 1919, to overcome the handicap of poor reagent chemicals, representatives of iiidustry, together with representatives of reagent users, undertook jointly under the sponsorship of the American Chemical Society, the task of establis...

the heterogeneous photo-fenton degradation of crystal violet under visible light has been investigated usingcopper modified iron oxide. the photocatalyst has been prepared by coprecipitation method. the rate ofphotocatalyic degradation of dye was monitored spectrophotometrically. it has been observed thatphotocatalytic degradation follows pseudo first order kinetics. the effect of various param...

Escherichia coli 30 S ribosomal subunits and 70 S ribosomes were treated with the bifunctional reagent diepoxybutane, acting as a cross-linker. One major cross-linked protein pair in the 30 S subunit was generated in relatively high yields. This cross-link was shown to consist of ribosomal proteins S13 and S19. Purification of this complex was achieved by a series of conventional and/or high pr...

CENTA is a newly synthesized, beta-lactamase-labile, chromogenic cephalosporin reagent which changes color from light yellow (K maximum ca. 340 nm) to chrome yellow (A maximum ca. 405 nm) concomitant with hydrolysis of the betalactam ring. This compound offers promise as a diagnostic reagent comparable to other chromogens (PADAC and nitrocefin) for the early detection of betalactamase-producing...

There are few reports concerning the epidemiology of Eimeria praecox and Eimeria mitis in Brazil. In the present experiment, the polymerase chain reaction (PCR) was used to identify these species in 156 samples of broiler chicken feces from several Brazilian states and the Federal District. Oocysts present in feces samples were purified by sodium chloride flotation followed by addition of DNAzo...

tripropylammonium fluorochromate(vi) (tpafc), is an efficient and new reagent, which isprepared easily and oxidizes thiols to the corresponding disulfides, quickly. the reactions areperformed cleanly and are controlled to stop at the disulfide stage, without over-oxidation andside products. coupling of thiols to their corresponding disulfides, are studied in solution atroom temperature and in s...