❆❜str❛❝t ✕ ❆ss❡ss✐♥❣ s♦❢t✇❛r❡ q✉❛❧✐t2 ❛ttr✐❜✉t❡s ✭s✉❝❤ ❛s ♣❡r❢♦r♠❛♥❝❡✱ r❡❧✐❛❜✐❧✐t2✱ ❛♥❞ s❡❝✉r✐t2✮ ❢r♦♠ s♦✉r❝❡ ❝♦❞❡ ✐s ♦❢ t❤❡ ✉t♠♦st ✐♠♣♦rt❛♥❝❡✳ ❚❤❡ ♣❡r❢♦r♠❛♥❝❡ ♦❢ ❛ s♦❢t✇❛r❡ s2st❡♠ ❝❛♥ ❜❡ ✐♠♣r♦✈❡❞ ❜2 ✐ts ♣❛r❛❧❧❡❧ ❛♥❞ ❞✐str✐❜✉t❡❞ ❡1❡❝✉t✐♦♥✳ ❚❤❡ ❛✐♠ ♦❢ t❤❡ ♣❛r❛❧❧❡❧ ❛♥❞ ❞✐str✐❜✉t❡❞ ❡1❡❝✉t✐♦♥ ✐s t♦ s♣❡❡❞ ✉♣ ❜2 ♣r♦✈✐❞✐♥❣ t❤❡ ♠❛1✐♠✉♠ ♣♦ss✐❜❧❡ ❝♦♥❝✉rr❡♥❝2 ✐♥ ❡1❡❝✉t✐♥❣ t❤❡ ❞✐str✐❜✉t❡❞ s❡❣♠❡♥ts✳ ■t ✐s ❛...

❆❜str❛❝t ✕ ❆ss❡ss✐♥❣ s♦❢t✇❛r❡ q✉❛❧✐t2 ❛ttr✐❜✉t❡s ✭s✉❝❤ ❛s ♣❡r❢♦r♠❛♥❝❡✱ r❡❧✐❛❜✐❧✐t2✱ ❛♥❞ s❡❝✉r✐t2✮ ❢r♦♠ s♦✉r❝❡ ❝♦❞❡ ✐s ♦❢ t❤❡ ✉t♠♦st ✐♠♣♦rt❛♥❝❡✳ ❚❤❡ ♣❡r❢♦r♠❛♥❝❡ ♦❢ ❛ s♦❢t✇❛r❡ s2st❡♠ ❝❛♥ ❜❡ ✐♠♣r♦✈❡❞ ❜2 ✐ts ♣❛r❛❧❧❡❧ ❛♥❞ ❞✐str✐❜✉t❡❞ ❡1❡❝✉t✐♦♥✳ ❚❤❡ ❛✐♠ ♦❢ t❤❡ ♣❛r❛❧❧❡❧ ❛♥❞ ❞✐str✐❜✉t❡❞ ❡1❡❝✉t✐♦♥ ✐s t♦ s♣❡❡❞ ✉♣ ❜2 ♣r♦✈✐❞✐♥❣ t❤❡ ♠❛1✐♠✉♠ ♣♦ss✐❜❧❡ ❝♦♥❝✉rr❡♥❝2 ✐♥ ❡1❡❝✉t✐♥❣ t❤❡ ❞✐str✐❜✉t❡❞ s❡❣♠❡♥ts✳ ■t ✐s ❛...

❆❜str❛❝t ✕ ❆ss❡ss✐♥❣ s♦❢t✇❛r❡ q✉❛❧✐t2 ❛ttr✐❜✉t❡s ✭s✉❝❤ ❛s ♣❡r❢♦r♠❛♥❝❡✱ r❡❧✐❛❜✐❧✐t2✱ ❛♥❞ s❡❝✉r✐t2✮ ❢r♦♠ s♦✉r❝❡ ❝♦❞❡ ✐s ♦❢ t❤❡ ✉t♠♦st ✐♠♣♦rt❛♥❝❡✳ ❚❤❡ ♣❡r❢♦r♠❛♥❝❡ ♦❢ ❛ s♦❢t✇❛r❡ s2st❡♠ ❝❛♥ ❜❡ ✐♠♣r♦✈❡❞ ❜2 ✐ts ♣❛r❛❧❧❡❧ ❛♥❞ ❞✐str✐❜✉t❡❞ ❡1❡❝✉t✐♦♥✳ ❚❤❡ ❛✐♠ ♦❢ t❤❡ ♣❛r❛❧❧❡❧ ❛♥❞ ❞✐str✐❜✉t❡❞ ❡1❡❝✉t✐♦♥ ✐s t♦ s♣❡❡❞ ✉♣ ❜2 ♣r♦✈✐❞✐♥❣ t❤❡ ♠❛1✐♠✉♠ ♣♦ss✐❜❧❡ ❝♦♥❝✉rr❡♥❝2 ✐♥ ❡1❡❝✉t✐♥❣ t❤❡ ❞✐str✐❜✉t❡❞ s❡❣♠❡♥ts✳ ■t ✐s ❛...

❆❜str❛❝t ✕ ❆ss❡ss✐♥❣ s♦❢t✇❛r❡ q✉❛❧✐t2 ❛ttr✐❜✉t❡s ✭s✉❝❤ ❛s ♣❡r❢♦r♠❛♥❝❡✱ r❡❧✐❛❜✐❧✐t2✱ ❛♥❞ s❡❝✉r✐t2✮ ❢r♦♠ s♦✉r❝❡ ❝♦❞❡ ✐s ♦❢ t❤❡ ✉t♠♦st ✐♠♣♦rt❛♥❝❡✳ ❚❤❡ ♣❡r❢♦r♠❛♥❝❡ ♦❢ ❛ s♦❢t✇❛r❡ s2st❡♠ ❝❛♥ ❜❡ ✐♠♣r♦✈❡❞ ❜2 ✐ts ♣❛r❛❧❧❡❧ ❛♥❞ ❞✐str✐❜✉t❡❞ ❡1❡❝✉t✐♦♥✳ ❚❤❡ ❛✐♠ ♦❢ t❤❡ ♣❛r❛❧❧❡❧ ❛♥❞ ❞✐str✐❜✉t❡❞ ❡1❡❝✉t✐♦♥ ✐s t♦ s♣❡❡❞ ✉♣ ❜2 ♣r♦✈✐❞✐♥❣ t❤❡ ♠❛1✐♠✉♠ ♣♦ss✐❜❧❡ ❝♦♥❝✉rr❡♥❝2 ✐♥ ❡1❡❝✉t✐♥❣ t❤❡ ❞✐str✐❜✉t❡❞ s❡❣♠❡♥ts✳ ■t ✐s ❛...

Y chromosome polymorphisms such as microsatellites or single nucleotide polymorphisms represent a paternal counterpart to mitochondrial DNA (mtDNA) for evolutionary and phylogeographic studies. The use of Y chromosome haplotyping in natural populations of species other than humans is still hindered by the lack of sequence information necessary for polymorphism screening. Here we used representa...

We present a novel methodology to determine the phase of single-nucleotide polymorphisms (SNPs) on a chromosome, which we term clone-based systematic haplotyping (CSH). The CSH procedure is based on separating the allelic chromosomes of a diploid genome by fosmid/cosmid cloning, and subsequent SNP typing of 96 clone pools, each representing approximately 10% of the genome. The pools are screene...

This article presents a six-rule algorithm for the reconstruction of multiple minimum-recombinant haplotype configurations in pedigrees. The algorithm has three major features: First, it allows exhaustive search of all possible haplotype configurations under the criterion that there are minimum recombinants between markers. Second, its computational requirement is on the order of O(J(2)L(3)) in...

Microsatellites are multi-allelic and composed of short tandem repeats (STRs) with individual motifs composed of mononucleotides, dinucleotides or higher including hexamers. Next-generation sequencing approaches and other STR assays rely on a limited number of PCR amplicons, typically in the tens. Here, we demonstrate STR-Seq, a next-generation sequencing technology that analyses over 2,000 STR...

Two new species of the cyrtoscydmine ant-like stone beetle genus Syndicus are described: S. (s. str.) birmanicus sp. n. (from Myanmar) and S. (Semisyndicus) chiangmaianus sp. n. (from Thailand). A previously unknown male of S. (s. str.) puncticollis Jałoszyński is described, and for the first time the spermatheca of S. (Semisyndicus) sumatrensis Schaufuss is illustrated. Syndicus puncticollis, ...

Stromelysin-3 (STR-3) is a recently characterized matrix metalloproteinase i MMI'i that was cloned on the basis of differential expression in benign and malignant breast tumors. This M Ml' has a unique processing mechanism and substrate specificity. Unlike previously characterized MMi's that are secreted as inactive zymogens, STR-3 is processed within the constitutive secretory pathway and secr...