‘J’HE MOST COMMONLY USED METHODS for the estimation of uric acid are those colorimetric procedures utilizing the reduction of phosphotungstic acid (1-7). Since these procedures are not specific for uric acid, an enzymatic procedure utilizing uricase, which oxidizes uric acid to allantoin, has been employed by a number of investigators (8-12). The enzymatic method, dependent upon the absorption ...

Introduction: Taking into consideration the high impact of Diabetes Mellitus type II (DM II) on human's health, identification of risk factors to prevent its development seems essential. Further studies are needed to ensure the positive role of uric acid in the onset of diabetes. This study targeted at investigating the attributed risk of hyperuricemia for the onset of DM II. Methods: In the p...

Previous studies have reported that uric acid stimulates vascular smooth muscle cell (VSMC) proliferation in vitro. We hypothesized that uric acid may also have direct proinflammatory effects on VSMCs. Crystal- and endotoxin-free uric acid was found to increase VSMC monocyte chemoattractant protein-1 (MCP-1) expression in a time- and dose-dependent manner, peaking at 24 hours. Increased mRNA an...

Several studies have reported that hyperuricemia is associated with the development of hypertension and cardiovascular disease. Increasing evidences also suggest that hyperuricemia may have a pathogenic role in the progression of renal disease. Paradoxically, uric acid is also widely accepted to have antioxidant activity in experimental studies. We aimed to investigate the association between g...

We assessed whether red cell distribution width (RDW) is associated with serum uric acid (UA) level in a group of 512 patients with newly diagnosed hypertension, recruited in Beijing. Patients were divided into high uric acid group and low uric acid group according to the median (334.9 μmol/L) of serum uric acid. Compared with the low uric acid group, the patients with high uric acid had higher...

Tumor lysis syndrome (TLS) is a life-threatening complication of cancer chemotherapy in which hyperuricemia is the principal characteristic. High serum uric acid levels can lead to uric acid precipitation in the renal tubules, which results in acute kidney failure. TLS is typically associated with rapidly dividing cell types observed with high-risk leukemia and lymphoma, which are more prone to...

Established methods for determining the serum uric acid concentration are colorimetric, enzymatic, or combination of the two. An alternative method, based on the affinity of purines for polyacrylamide resin, has been developed. Uric acid, more strongly adsorbed than other major serum constituents, elutes later (with phosphate buffer, pH 7) from a poly. acrylamide column. The separated serum uri...

The relation between creatinine and uric acid metabolism was analysed in 77 male patients with primary gout and 62 healthy male subjects. Significant positive correlations between 24 hour urinary creatinine and uric acid excretion were shown in both groups. The mean urinary creatinine and uric acid excretions in the patients with gout were significantly increased as compared with those of norma...

Gout is a common inflammatory arthritis that is caused by chronically-elevated serum uric acid (sUA) levels (hyperuricemia). In humans, sUA levels are predominantly controlled by a variety of transporters that mediate the elimination of uric acid through the kidneys and intestines, a process that is altered in most gout patients. In this review, we highlight our current understanding of uric ac...