We previously reported the activation of adenylate cyclases from rat brain (Johnson, R. A., Awad, J. A., Jakobs, K. H., and Schultz, G., (1983) FEBS Lett. 152, 11-16) and from human platelets (Jakobs, K. H., Johnson, R. A., and Schultz, G. (1983) Biochim. Biophys. Acta 756, 369-375) by a factor derived from bovine sperm. In this report we describe the conditions for the extraction of the factor...

The aim of this study was to evaluate the effect ethanol leaf extract V. amygdalina on testicle alloxan induced diabetic wistar rats. Freshly harvested leaves washed with clean tap water and subsequently dried at room temperature prior being ground fine powder. Twenty (20) adult male rats were divided into four (4) groups five (5) per group. · Group I normal control fed rat chow II diabetes wit...

introduction: the purpose of this research was to study the effects of two cryoprotectants on viability, motility, morphology and fertilization rate of mouse sperm after freezing-thawing. material and methods: spermatozoa were isolated from caudae epididymis of nmri male mice (8 to 10 weeks-old) and divided to three groups of control, experimental 1 and 2. cryoprotectant solution for the first ...

Cysteine-rich secretory protein-1 (CRISP-1) is a glycoprotein secreted by the epididymal epithelium. It is a member of a large family of proteins characterized by two conserved domains and a set of 16 conserved cysteine residues. In mammals, CRISP-1 inhibits sperm-egg fusion and can suppress sperm capacitation. The molecular mechanism of action of the mammalian CRISP proteins remains unknown, b...

The ZyMōt sperm separation device has proven favorable for use in elevated DNA fragmentation index (DFI) male factor patients, as an alternative to density gradient (DG) washing or surgically attained testicular sperm. In 2020, without fully understanding the infectivity and transmission potential of SARS-CoV2 semen, a more liberal application timed microfluidic swim-up was applied our IVF pati...

Regulation of estrus in the ewe with prostaglandin or progestogen causes death of sperm in the cervix and inhibits sperm transport through the cervix at the regulated estrus. Because removal of the corpus luteum (CL) alters the estrous cycle, experiments were conducted to determine the effect of removing the CLbearing ovary on sperm survival and transport in the cervix at the ensuing estrus. In...

The advances in treatment of HIV and the introduction of polymerase chain reaction assay for the virus now make it acceptable for HIV discordant couples where the male partner is seropositive to attempt to conceive through artificial insemination by husband (AIH) or via in vitro fertilisation. With undetectable viral load and washed sperm, there is minimal risk of transmission of HIV to the fem...

This study assesses the fertilization and blastocyst-forming rate in mice cumulus-oocyte complexes (COCs) after the exposure of human ovarian endometriotic fluid. Endometriotic fluid was obtained from a single patient by aspiration at the time of a laparoscopic cystectomy and serially diluted. COCs were obtained from 46-week-old female BDF1 mice. After exposure to ovarian endometriotic fluid fo...

The efficiency of sperm capacitation and of the acrosome reaction was studied in the teratospermic domestic cat to evaluate further the etiology of compromised zona pellucida penetration and oocyte fertilization. Specific objectives were to compare normospermic and teratospermic cat ejaculates for 1) the kinetics and timing of sperm capacitation in vitro as determined by an ionophore-induced ac...