Many proteins that accumulate in the form of insoluble aggregates when they are overproduced in Escherichia coli can be rendered soluble by fusing them to E. coli maltose binding protein (MBP), and this will often enable them to fold in to their biologically active conformations. Yet, although it is an excellent solubility enhancer, MBP is not a particularly good affinity tag for protein purifi...

A surface plasmon resonance imaging-based Ni(2+)-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.

Fluorescent probes are essential for the exploration of protein function, detection of molecular interactions, and conformational changes. The nitrilotriacetic acid derivatives of different chromophores were successfully used for site-selective noncovalent fluorescence labeling of histidine-tagged proteins. All of them, however, suffer from the same drawback--loss of the fluorescence upon bindi...

Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, S...

Chondroid syringoma miks kutanöz tümör olarak adlandırılan benign karakterde bir cilt tümörüdür. Chondroid syringoma klinik olarak yavaş büyüyen, ağrısız, ülsere olmayan, subkutanöz veya intrakutanöz 0,5 ila 3 cm arasında boyutlara sahip nodul şeklinde görülür. Chondroid syringoma genellikle baş boyun bölgesinde orta yaş ve yaşlı erkeklerde görülür. Nadir olarak gövde, sırt, ekstremiteler ve ge...

Herman F. Mark (1895–1992) was an Austrian-born American pioneer of polymer chemistry. He made substantial discoveries in other areas chemistry as well. One them 1930, when he and his physicist associate, Raimund Wierl, initiated the gas-phase electron diffraction technique molecular structure determination. This article follows Mark’s path to establishing new technique, presents circumstances ...

Professor Sykes’s elegant article explains with great clarity how rational self-interest alone can both justify and explain states’ compliance with international agreements. Such agreements will effectively constrain the behavior of states when all states involved stand to gain from cooperation and some effective enforcement mechanism is available. Crucial to his argument is that enforcement do...