In this study, we established an in-house database of yeast internal transcribed spacer (ITS) sequences. This database includes medically important as well as colonizing yeasts that frequently occur in the diagnostic laboratory. In a prospective study, we compared molecular identification with phenotypic identification by using the ID32C system (bioMérieux) for yeast strains that could not be i...

The aim of the study was to evaluate the accuracy of CHROMagar-Candida for the identification of Candida spp. from different clinical specimens. The growth of cultures on plates, containing Sabouraud dextrose agar and CHROMagar-Candida were compared. Non-Candida albicans yeast isolates were further specificated with API 20C (bioMerieux, France). Of the 178 cultures processed, 100% accuracy for ...

CHROMagar, a chromogenic differential culture medium, is claimed to facilitate the isolation and presumptive identification of certain clinically important yeast species, e.g., Candida albicans. This study evaluated the cost-effectiveness and time advantage of using it in comparison with Sabouraud dextrose agar (SDA). Three possible pathways, each of which included the use of one or both media,...

Abstract In this study, 14 yeast cultures from 62 isolates traditional sourdoughs collected 6 different regions of Turkey were selected by FT-IR identification and characterised to reveal their probiotic properties. Four strains genotypically identified compared with identification. all analyses, it was observed that mostly Saccaromyces cerevisiae strain exhibited high hydrophobicity, auto-aggr...

The Uni-Yeast-Tek system, a commercially prepared kit and scheme for the rapid identification of medically important yeasts (Corning Medical), was evaluated in comparison with a conventional procedure in the identification of 623 yeasts. The system permitted the presumptive identification of 99.8% of 436 isolates representing 16 common species commonly isolated in the clinical laboratory. Corre...

Hop-derived volatile organic compounds (VOCs) and their transformation products significantly impact beer flavour aroma. Geraniol, a key monoterpene alcohol in hops, has been reported to undergo yeast-modulated biotransformation into various terpenoids during fermentation, which impacts the citrus floral aromas of finished beer. This study monitored evolution geraniol its throughout fermentatio...

Evidence indicated an accumulation of l-malic acid in the synthetic growth medium of both lysine auxotrophs and wild-type yeast. Malic acid was isolated from growth experiments and was identified by paper and column chromatography, by comparison of infrared spectra with authentic l-malic acid, as well as enzymatically, as a substrate of malic dehydrogenase. Malic acid was accumulated when gluco...

One focus of postgenomic research concerns the regulation of gene expression through signal transduction and combinatorial control of gene transcription. Protein-protein interactions and protein-DNA interactions provide the dynamic backbones of regulatory networks which define the complex response of a cell to different environmental conditions. More recently, large scale experiments have resul...