This study evaluates the use of two fluorescein-labelled (FITC) plant lectins, Pisum sativum (edible pea) agglutinin (PSA) and Arachis hypogaea (peanut) agglutinin (PNA), in order to determine the most accurate and reliable method to experimentally detect and assess the acrosome reaction in mouse spermatozoa. PNA-FITC labelling was restricted to the acrosome and was not influenced by the fixati...

Evidence has accumulated suggesting multiple roles of acrosin in fertilization, including its participation in early steps of gamete recognition and binding. However, the implication of acrosin in many of these processes is not compatible with its presumptive sequestration within the sperm acrosome until a late phase of the acrosome reaction. In an earlier study (J. Tesarik, J. Drahorad, J. Pek...

The study evaluated the effect of two animal protein-free extenders on ram semen cryopreservation tropical hair breeds. ejaculates were collected from 8 rams (4 Blackbelly and 4 Pelibuey), which mixed (pooled) by breed, diluted cryopreserved in three different extenders: Animal protein [Tris egg yolk (Triladyl®)], AndroMed® (lecithin soy bean) OPTIXcell® (liposome). Sperm analyses total (TM) pr...

OBJECTIVES Capacitation and acrosome reaction are prerequisites for fertilization. However, in vitro capacitation is not necessary for an agonist-induced acrosome reaction. We studied whether in vitro capacitation is important in spontaneous acrosome reaction and analyzed how capacitation before cryopreservation influences the acrosomal status of thawed spermatozoa. METHODS Semen specimens fr...

In this study, buffalo spermatozoa were in-vitro capacitated and acrosome reacted using modified Tyrode’s bicarbonatebuffered medium (sp-TALP media). Lipids were extracted from freshly ejaculated, in-vitro capacitated and acrosome reacted buffalo spermatozoa. The total lipid content of freshly ejaculated spermatozoa (171.5±2.90 μg/108 cells), capacitated spermatozoa (128.2±3.29 μg/108 cells) an...

REČKOVá, Z., MACHATKOVá, M., RyBář, R., MáCHAL, L.: Monitoring of chromatin integrity changes in the population of motile bovine sperm capacitated in vitro. Acta univ. agric. et silvic. Mendel. Brun., 2007, LV, No. 2, pp. 65–70 The objective of our study was to standardize a method for chromatin integrity assessment in a separated population of bovine sperm and monitor the changes occurring dur...

There are still concerns over sperm quality when plant-based diluents used instead of animal-based semen extenders. Therefore, in our study we compared the effects one soybean lecithin-based (AndroMed®) and two egg yolk-based (BullXcell® Optidyl®) commercially available extenders on post-thaw vitro quality. Fifty ejaculates collected from ten bulls were aliquoted into three parts diluted with a...

The spermatozoa of the musk shrew, Suncus murinus, have a fan-like giant acrosome with a diameter of approximately 20 mm. The aim of this study was to investigate how this giant acrosome is constructed in the musk shrew spermatid and, in particular, how the Golgi apparatus involved in acrosome formation behaves. The behaviour of the Golgi apparatus was monitored by confocal laser scanning micro...

In a prospective, blind study, we have examined the relationship among the expression of human sperm surface progesterone receptors, the ability to undergo a mannose-stimulated acrosome reaction and the rate of fertilization in vitro. Individual aliquots of motile spermatozoa were surface-labelled with progesterone and/or mannose-fluoresceinated ligands. Spontaneous acrosome loss and the increa...

The effect of pretreatment of rabbit sperm cells with different concentrations of cholesterol-loaded cyclodextrin (CLC) on the occurrence of premature acrosome reactions during 72 h of liquid storage was investigated in three successive experiments. The aim of the first experiment was to establish a liquid storage model to facilitate premature acrosome reactions in rabbit sperm cells and, there...