OBJECTIVE Growth factors demonstrate mixed results improving wound healing. Amnion-derived multipotent cells release physiologic levels of growth factors and tissue inhibitors of metalloproteinases. This solution was tested in models of acute and chronic wound healing. METHODS Acute model: Sprague-Dawley rats underwent laparotomy incisions. The midline fascia was primed with phosphate-buffere...

Scaffolds derived from decellularized tissues provide a natural microenvironment for cell culture. Embryonic cerebrospinal fluid (e-CSF) contains factors which play vital roles in development of the nervous system. This research was aimed to survey the effect of Wistar rat e-CSF on neural differentiation of bone marrow derived mesenchymal stem cells (BM-MSCs) cultured on the human amniotic memb...

OBJECTIVE To establish a safety profile for amnion-derived cellular cytokine solution following topical application in patients undergoing whole breast radiotherapy for breast cancer. MATERIALS AND METHODS Twenty female patients with early-stage breast cancer were enrolled in 2 separate cohorts of an institutional review board-approved phase I protocol. Cohort 1 consisted of 10 patients who r...

Pre-term birth is the leading cause of perinatal and neonatal mortality, 40% of which are attributed to the pre-term premature rupture of amnion. Rupture of amnion is thought to be associated with a corresponding decrease in the extracellular collagen content and/or increase in collagenase activity. However, there is very little information concerning the detailed organisation of fibrillar coll...

Objectives: To evaluate the amnion graft in vaginoplasty, to assess the advantage of a flap placed over fourchette and to evaluate the benefit of gauze mould. Methods: Out of the 35 subjects of primary amenorrhea, eight with complete vaginal agenesis were selected for vaginoplasty. Half of these received split thickness skin graft and other half received amnion graft. An additional triangular f...

Background: Split-thickness skin graft (STSG) covers patient’s primary wound, but, at the expense of a donor-wound which heals by secondary intention. This study evaluated efficacy amnion and calcium alginate dressings STSG donor sites in terms healing duration, patient comfort incidence infection.
 Patients methods: An open label randomised control trial was conducted during October 2018 ...

OBJECTIVE The proteoglycan decorin stabilizes collagen whereas biglycan and hyaluronan disrupt well-organized collagen. The aim was to compare hyaluronan and proteoglycans in human fetal membranes obtained before and after spontaneous labour at term. STUDY DESIGN Prelabour samples of fetal membranes (N=9) were obtained from elective caesarean sections and regionally sampled from over the cerv...