BACKGROUND JAK2 V617F, a somatic point mutation that leads to constitutive JAK2 phosphorylation and kinase activation, has been incorporated into the WHO classification and diagnostic criteria of myeloid neoplasms. Although various approaches such as restriction fragment length polymorphism, amplification refractory mutation system and real-time PCR have been developed for its detection, a gene...

Early detection of epidermal growth factor receptor (EGFR) mutation, particularly EGFR T790M mutation, is of clinical significance. The aim of the present study was to compare the performances of amplification refractory mutation system-based quantitative polymerase chain reaction (ARMS-qPCR) and droplet digital polymerase chain reaction (ddPCR) approaches in the detection of EGFR mutation and ...

AIM To conduct developmental validation studies on a polymerase chain reaction (PCR) based short tandem repeat (STR) multiplex typing system, developed for the purpose of genetic individualization and parentage testing in domestic cat samples. METHODS To evaluate reproducibility of the typing system, the multiplex was amplified using DNA extracted from hair, blood, and buccal samples obtained...

schizophrenia is a sophisticated mental disability which has affected nearly1.1% of people all over the world. according to recent researches, the key proteins triggered in the immune system are cytokines which might also be taking part in the pathogenesis of schizophrenia. the aim of this study was to evaluate the relationship between the -1082g/a and +874t/a polymorphisms of il-10 and ifn-γ g...

introduction: colorectal cancer (crc) is one of the most prevalent cancers in the world. genetic disorders and dietary habits play a part in crc. the most important cancer suppressor gene is p53. different mutations rates in 7th exon of p53 gene are reported in different areas  which may have a relationship with prognosis of crc. this study examines the relationship between mutation in 7th exon...

In this study, slag penetration into a magnesia refractory monolithic was investigated by the crucible test method. A synthetic calcium aluminate slag system has been used to study commercial magnesia mix refractory for 1, 2, 3, 4, 5 and 6 hours at 1450°C and 1600°C. It has been shown that the penetration rate is controlled by a diffusion mechanism at 1450°C. In this case, capillaries are the ...

Abstract Objective The NUDT15 variants impact thiopurine dose selection in acute lymphoblastic leukemia patients. ability to rapidly detect is important clinical practice. This study aims develop a simple polymerase chain reaction (PCR) procedure for detecting Vietnamese Materials and Methods Sanger sequencing was used determine from 200 We designed primers optimized the PCR detection of wild-t...

objective(s): the major antibiotic efflux pump of esherichia coli is acrab-tolc. the first part of the pump, acrab, is encoded by acrab operon. the expression of this operon can be kept elevated by overexpression of an activator, mara following inactivation of marr and acrr repressors due to mutation in encoding genes, marr and acrr, respectively. the aims of this research were to use e. coli m...

Resistance to trastuzumab is frequently observed during the treatment of patients with human epidermal growth factor 2 (HER2)-positive metastatic breast cancers. The aim of the present study was to determine if the phosphorylated proline-rich Akt substrate of 40 kDa (phospho-PRAS40Thr246), a novel biomarker for phosphoinositol-3 kinase (PI3K) pathway activation, could predict the response of HE...

Abstract Background Von Willebrand disease (VWD) is an autosomal recessive congenital bleeding disorder with deficiency or dysfunction of von Willebrand factor (VWF). The gene encoding for the VWF is located on chromosome 12, which is 178 Kb with 52 exons. Various mutations of this gene is responsible for the clinical features of VWD, but some single nucleotide polymorphisms make the molecu...