Abstract Radiotherapy resistance is a challenge for colorectal cancer (CRC) treatment. Circular RNAs (circRNAs) play vital roles in the occurrence and development of CRC. This study aimed to investigate role circ_0005615 regulating radiosensitivity The levels circ_0005615, microRNA-665 (miR-665), notch receptor 1 (NOTCH1) were detected by quantitative real-time PCR or western blot. CRC cells wa...

Introduction The aim of this study was to discuss the effects and mechanisms lncRNA in renal injury induced by diabetes vivo vitro study. Material methods Collecting healthy DN patients, measuring ANRIL expression RT-qPCR assay serum. Measuring normal, 10d 20d model rats kidney. LDH, MDA, SOD, IL-6 TNF-α concentration Elisa assay, evaluating kidney pathology HE staining, cell apoptosis TUNEL re...

the objective of this study was to observe the apoptosis-inducing effect and mechanism of baicalin on human cervical cancer hela cells. the inhibitory effect of baicalin on the growth of hela cells was measured by mtt assay, and cell proliferation and migration was analyzed by cell scratch assay. morphological changes of apoptotic cells were viewed by the light microscope and electron microscop...

Cancer remains to be a major global healthcare burden as 18.1 million new cases and 96 death occurred 2018 on the basis of report from international agency for research cancer Breast is most common diagnosed in women causing highest morbidity. Ipomoea carnea leaf extracts have been used chemotherapeutic agent due their antioxidant, anti-inflammatory, anti-bacterial, anti-tumor effect. The aim s...

Gastric carcinoma has a high incidence, accounting for approximately 6% of all cancers worldwide. The in vivo antitumor effect sodium selenite on gastric been demonstrated. This study therefore aimed to further explore its targets cancer vitro and elucidate mechanism action. effects inorganic (Na2SeO3) apoptosis, proliferation, invasion cells were investigated, the interaction between Na2SeO3 e...

Abstract Background Long non-coding RNA is considered to be essential modulate the development and progression of human malignant cancers. And long can act as crucial modulators by sponging corresponding microRNA in tumorigenesis. We aimed elucidate function ACTA2-AS1 its molecular mechanism colon adenocarcinoma. Materials methods The expression ACTA2-AS1, miR-4428 BCL2L11 adenocarcinoma tissue...

Piperine is a dietary alkaloid with huge pharmacological importance and was first isolated from pepper extract. It has been reported anti-cancer, anti-amoebic, anti-mycobacterial, anti-mutagenic, anticonvulsant, anti-asthmatic, immunomodulatory, anti-inflammatory anti-oxidant activity. Therefore, the current investigation designed to unveil anticancer effects of piperine molecule against human ...

Chemotherapeutic agents exert their antitumor effects by inducing apoptosis. The microculture kinetic (MiCK) assay provides an automated, continuous means of monitoring apoptosis in a cell population. We used the MiCK assay to determine the chemosensitivities of the human promyelocytic HL-60 and lymphoblastic CEM cell lines and leukemia cells freshly isolated from patients with acute nonlymphoc...

the cytotoxic activity of natural killer cells is usually tested by radioactive assay csi cr release assay), which detects the release of cytoplasmic contents after plasma membrane disintegration of dying cells. in contrast to this indirect evaluation of cytotoxicity, the assessment of cell damage by flow cytometry aims to provide a more exact characterization of the death pathway via detection...