Purpose. To optimize the method of clonal micropropagation rocket (Eruca sativa Mill.). Methods. Seeds cultivars 'Znahar' and 'Lybid' were used for introduction into in vitro culture. Solutions sodium hypochlorite (35%), ethanol (70%) HgCl2 (0.2%) sterilization. A 5% solution chloramine served as a control. Sterile material was planted on liquid solid agar nutrient media according to Murashige-...

BACKGROUND AND AIMS Cassava (Manihot esculenta) has three adventitious root types: primary and secondary fibrous roots, and storage roots. Different adventitious root types can also regenerate from in vitro cultured segments. The aim of this study was to investigate aspects of in vitro production of storage roots. METHODS Morphological and anatomical analyses were performed to identify and di...

The cucumber cotyledon greening bioassay is frequently used for detecting cytokinins. Beneficial modifications of the original technique included using 5-day-old cucumber (Cucumus sativus L., cv. National Pickling) cotyledons treated with combinations of 40 millimolar KCl and various concentrations of cytokinins. A dark incubation period of 20 hours was followed by an exposure to light for 3.5 ...

Cinchona officinalis is an important species from the Andean cloud forest that has a low regeneration rate in natural populations. In vitro of C. been successfully established but somaclonal variation was not evaluated. The pathway and number subcultures on were evaluated using six ISSR primers amplified 58 loci Inter Simple Sequence Repeats (ISSR). A dendrogram based Jaccard´s genetic distance...

Eucalyptus gunnii Hook microcuttings, obtained in vitro through axillary bud stimulation, show different rooting responses on the same rooting medium depending on the physiological state induced by cytokinins used in the previous multiplication medium. 6-Furfurylamino purine and 6-(4-hydroxy-3-methylbut-2-enylamino)purine induced a physiological state characterized by high sensitivity of microc...

A protocol has been devised for enhanced in vitro regeneration of critically endangered Mantisia spathulata Schult. and Mantisia wengeri Fischer. Highest Bud Forming Capacity (BFC) of 6.10 +/- 0.55 with an average of 19.93 +/- 3.19 roots was obtained for M. spathulata within 5-6 weeks in Murashige and Skoogs (MS) medium supplemented with a combination of 10.0 microM of N6-benzyladenine (BA) and...