human serum albumin (hsa) is an abundant, multifunctional and nonglycosylated negatively charged plasma protein. hsa ascribed ligand-binding and transport properties, antioxidant functions and enzymatic activities. in the present study, the interaction and side effects of a new designed anti-cancer compound (1,10-phenanthroline butyl dithiocarbamato palladium(ii) nitrate) on hsa have been inves...

In depth interaction studies between calf thymus deoxyribonucleic acid (CT-DNA) and a series of four structurally relative palladium(II) complexes [Pd(en)(HB)](NO3)2 (a-d), where en is ethylenediamine and heterocyclic base (HB) is 2,2'-bipyridine (bpy, a); 1,10-phenanthroline (phen, b); dipyridoquinoxaline (dpq, c) and dipyridophenazine (dppz, d) (Figure 1), were performed. These studies have b...

linear–dendrite copolymers containing hyper branched poly(citric acid) and linear poly(ethylene glycol) blocks pca–peg–pca are promising nonmaterial to use  in nanomedicine. to investigate their potential application in biological systems (especially for drug carries) oniom2 calculations were applied to study the nature of particular interactions between drug and the polymeric nanoparticles.  b...

the binding of drugs by plasma proteins is an important phenomenon, because it influences the size of the free fraction of the drugs in plasma. in this study the influence of dipyridamole (dp) on the protein binding of propranolol (pl) and pl on the protein binding of dp were studied alone and in the presence of nicotine (nc).        the equilibrium dialysis was employed for protein binding stu...

background: acanthamoeba keratitis develops by pathogenic acanthamoeba such as a. pal­es­tinen­sis . indeed this species is one of the known causative agents of amoebic keratitis in iran. mannose binding protein (mbp) is the main pathogenicity factors for developing this sight threatening disease. we aimed to characterize mbp gene in pathogenic acanthamoeba isolates such as a. palestinensis . m...

immune genes of the major histocompatibility complex (mhc) are among the most polymorphic genes in the vertebrate genome. due to their polymorphic nature, they are often used to assess the adaptive genetic variability of natural populations. this study describes the first molecular characterization of 13 partial mhc class iib sequences from three european ranid frogs.  the utility of previously...

Horseradish peroxidase (HRP), has gained significant interests in biotechnology, especially in biosensor field and diagnostic test kits. Hence, its solvent-exposed lysine residues 174, 232, and 241 have been frequently modified with the aim of improving its stability and catalytic efficiency. In this computational study, we investigated the effects of Lys-to-Glu substitutions on HRP structure t...