conclusions according to the results of the present study, it can be concluded that adipose derived mscs can be cultured in serum-free media with no change in their differentiating capacity. this finding gives us a hope for future cell therapy studies and trials with little concern about zoonotic infections or immunological reaction. results the isolated cells showed adherent spindle shaped mor...

background and objectives: the seeds of securigera securidaca (l.) deg. & dorf. (fabaceae) are used as anti-diabetic remedy in iranian folk medicine. the aim of the present study was to establish the callus and suspension culture of s. securidaca seeds for the first time and to determine the major secondary metabolites including cardiac glycosides and total phenols. methods: the culture of s. s...

cell cultures of varthemia persica dc. have been studied to evaluate their abilities in biotransformation of aromatic and aliphatic precursors. v. persica (asteraceae) is an aromatic plant growing in iran. v. persica contain different terpens but its cell culture does not posses these compounds. callus cultures of v. persica was established from seedlings and healthy suspensions were grown usin...

Background and Aims: It has been proven that human mesenchymal stem cells (MSCs) conditioned medium (hMSCs-CM) can influence human embryonic stem cells (hESCs) chondrogenic differentiation. In this study, we hypothesized that conditioned medium (CM) from hESCs-derived MSCs in a sequential 3D-2D culture system could facilitate the induction of chondrogenesis in hESCs. Materials and Methods: CM ...

background and objectives : each year, enteroviruses infect millions of people and cause different diseases. the agents are usually detected using cell culture. rd (rhabdomyosarcoma) and l20b (l cells) are among the recommended cells by the world health organisation (who) for this purpose. even though cell culture is the most common method used in diagnosing enteroviruses in stool specimens, th...

Control of carbon dioxide within the optimum range is important in mammalian bioprocesses at the manufacturing scale in order to ensure robust cell growth, high protein yields, and consistent quality attributes. The majority of bioprocess development work is done in laboratory bioreactors, in which carbon dioxide levels are more easily controlled. Some challenges in carbon dioxide control can p...

introduction: this study was initiated to examine the effect of the ampullary and the isthmic primary cell cultures of human oviduct during long-term co-culture on two-cell mouse embryos. materials and methods: using a mechanical procedure, epithelial cells from ampullary (a) and isthmic (i) regions of the human oviduct were isolated and cultured in ham's f-10 with 10% fetal calf serum (fsc). t...

introduction: it is now generally accepted that vero cells provide beneficial effects on improving embryo quality in many animal species. the aim of this research was to determine the effect of vero cell co-culture on the development of vitrified 8-cell mouse embryos. material and methods: eight- cell mouse embryos were provided from superovulated female nmri mice and divided into treatment cas...