cellular and molecular pathways link thrombosis and innate immune system during sepsis. extrinsic pathway activation of protease thrombin through fviia and tissue factor (tf) in sepsis help activate its endothelial cell (ec) membrane protease activated receptor 1 (par-1). thrombin adjusts the ec cycle through activation of g proteins (g12/13), and later through rho gefs (guanine nucleotide exch...

toxicity bioassays are important tools to determine biological effects of chemical agents on species. the questions remained on, what effects have been imposed on each of the different molecular site of cells by chemical exposure and how to find a pattern for chemical toxicity. to address the questions, hepg2 cell lines were exposed to the different concentrations of cisplatin for 24 hours to r...

Modification of the 2-(acylamino)pyridine scaffold led to identification structure–activity relationships that improve RIOK2 cellular potency and maintain its selectivity.

Ubiquitination (a.k.a. ubiquitylation) is a protein post-translational modification that typically requires the sequential action of three enzymes: E1 (ubiquitin-activating enzymes), E2 (ubiquitin-conjugating and E3 (ubiquitin ligases) [28]. proteins can target them for proteasomal degradation, or modulate cellular processes including cell cycle progression, transcriptional regulation, DNA repa...

Cellular RNAs are chemically modified by many RNA modification enzymes; however, often the functions of modifications remain unclear, such as for pseudouridine formation in the tRNA TΨC arm by the bacterial tRNA pseudouridine synthase TruB. Here we test the hypothesis that RNA modification enzymes also act as RNA chaperones. Using TruB as a model, we demonstrate that TruB folds tRNA independent...