BACKGROUND The aim of this study was to evaluate the effects of vitrification on morpho-functional parameters (blastomere/chromatin integrity and bioenergy/oxidative potential) of mouse preimplantation embryos. METHODS In vivo produced mouse (4/16-cell, morulae and blastocyst-stage) embryos were randomly divided into vitrification and control groups. For vitrification, embryos were exposed to...

Background: Diabetes mellitus is a metabolic condition characterized by elevated blood glucose levels secondary to absolute impairment of insulin secretion. Maternal diabetes has been demonstrated to adversely affect pre implantation embryo development and pregnancy outcomes. Emerging evidence has implicated that these effects are associated with compromised oocyte competence. Several developme...

Abstract Study question How different cryoprotectants at various concentrations affect ice crystals formation and propagation during vitrification? Summary answer The velocity of crystal is inversely related to concentration type responsible for downstream cell damage vitrification. What known already Vitrification has replaced slow freezing oocyte embryo cryopreservation now being considered a...

The aim of this study was to evaluate the effects of ovarian tissue vitrification and two-step in vitro culture on the metaphase II (MII) oocyte reactive oxygen species (ROS) level, mitochondrial transcription factor A (TFAM) expression and succinate dehydrogenase (SDH) activity. After collection of neonatal mouse ovaries, 45 ovaries were vitrified and the others (n = 45) were...

The aim of this study was to evaluate the suitability of a commercial kit for bovine embryo vitrification for cryopreserving cat oocytes and to evaluate comparatively the effects of its use with slow freezing procedure on cryotolerance in terms of morphology and oocyte resumption of meiosis. Germinal vesicle stage oocytes isolated from cat ovaries were either vitrified (n = 72) using a vitrific...

OBJECTIVE To evaluate a cryopreservation technique by vitrification of whole ovaries with their vascular pedicle in sheep, by using two cryoprotectant solutions. DESIGN Animal study. SETTING Fertility clinic in a university teaching hospital. ANIMAL(S) Five to 6-month-old ewes. INTERVENTION(S) Whole sheep ovaries with their vascular pedicles were collected at the slaughterhouse and prep...

One of the several factors that contribute to the low efficiency of mammalian somatic cloning is poor fusion between the small somatic donor cell and the large recipient oocyte. This study was designed to test phytohemagglutinin (PHA) agglutination activity on fusion rate, and subsequent developmental potential of cloned bovine embryos. The toxicity of PHA was established by examining its effec...

BACKGROUND Vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. However, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. This study was carried out to explore and compare the effect(s) of vitrification on mouse 2-cell, 4-cell, 8-cell, mo...

The effects of vitrification on the embryo viability of the Persian sturgeon (Acipenser persicus) were investigated in this study. Vitrificant solutions (VS1-VS6) were prepared by combining propylene glycol (PG) as basic cryoprotectant plus other permeable and non-permeable cryoprotectants in different concentration. Embryos at neurulation stage were selected and incubated in six vitrificant so...