introduction: superovulation produces complications in some patients, so invitro maturation of oocytes is used to decrease or eliminate these complications and improve ivf. moreover, ivm is used for different aspects of reproductive researches. slaughterhouse ovaries are the main source of oocytes for ivm and ivf studies. different media has been introduced and experimented for in vitro maturat...

Oocyte maturation and embryo development are controlled by intra-ovarian factors such as steroid hormones. Progesterone (P4) exists in the follicular fluid that contributes to normal mammalian ovarian function and has several critical functions during embryo development and implantation, including endometrial receptivity, embryonic survival during gestation and transformation of the endometrial...

The present study was designed 1) to examine the influence of cumulus cells, ovary storage time and oocyte size on the penetrability of immature pig oocytes, and 2) to investigate the effect of 2 methods of treating the semen from different boars on the inter-assay variability of homologous in vitro penetration tests of boar sperm fertility. In Experiment 1, cumulus oocyte complexes, oocytes wi...

background:   objective: this study was designed to examine the effects of hp on in-vitro maturation of oocytes and cell death in cumulus cells derived from vitrified-warmed mouse ovaries.   materials and methods: preovulatory follicles were harvested from non-vitrified and vitrified-warmed 6-8 week-old female nmri mouse ovaries and randomly assigned to following groups: non-vitrified (control)...

BACKGROUND Linoleic acid (LA) is a polyunsaturated fatty acid present in high concentrations in follicular fluid, when added to maturation culture media, it affects oocyte competence. OBJECTIVE In the present study, we investigated effect of linoleic acid supplementation on in vitro maturation, embryo development and apoptotic related gene expression in ovine. MATERIALS AND METHODS The expe...

The aim of this study was to determine the quality of cumulus-oocyte-complexes (COCs) and the effects of bovine serum albumin (BSA) supplementation on in vitro maturation and fertilization rate of buffalo oocytes. COCs were collected from slaughterhouse buffalo ovaries by aspiration method. Only normal grades COCs were matured for 48 hours in TCM-199 media. Two groups were created: one for the ...

2012 School of Biological Science 2 Acknowledgements I would like to acknowledge, with appreciation, everyone who has contributed to this research. Firstly I would like to thank my primary supervisor Professor Ken McNatty for making it possible for me to do this research and for his patience and understanding in the face of some dumb moments on my part. I couldn't have completed this without hi...

background: in vitro maturation (ivm) of oocytes is a promising technique to reduce the costs and avert the side-effects of gonadotropin stimulation for in vitro fertilization (ivf). the pregnancy rates from oocytes matured in vitro are much lower than those of in vivo stimulation cycles, indicating that optimization of ivm remains a challenge. objective: in this study, we investigated the effe...

A better understanding of the paracrine and autocrine regulatory loops within the cumulus-oocyte complex (COC) is fundamental for the improvement of in vitro maturation (IVM) outcomes in humans and domestic species. This review presents the most important local regulators identified in the COC to date with special attention to those secreted by the oocyte and acting on cumulus cells, as well as...