Artificial DNA cutters have been developed by us in our previous studies by combining two strands of pseudo-complementary peptide nucleic acid (pcPNA) with Ce(IV)-EDTA-promoted hydrolysis. The pcPNAs have two modified nucleobases (2,6-diaminopurine and 2-thiouracil) instead of conventional A and T, and can invade double-stranded DNA to activate the target site for the scission. This system has ...

Tropical leaf-cutter ants cultivate the fungus Attamyces bromatificus in a many-to-one, diffuse coevolutionary relationship where ant and fungal partners re-associate frequently over time. To evaluate whether ant-Attamyces coevolution is more specific (tighter) in peripheral populations, we characterized the host-specificities of Attamyces genotypes at their northern, subtropical range limits (...

Curvilinear tooth gears are commonly produced on a face milling cutter and a hob cutter. However, this paper proposes a kind of curvilinear tooth gear which is processed by a parallel linkage with a single blade cutter. For the purpose of identifying the meshing and contact characteristics of curvilinear tooth gears, the paper covers the following: (i) equations of tooth surfaces are deduced fr...

BACKGROUND Despite the growing acceptance of laparoscopic colon surgery, an abdominal incision is needed to remove the specimen and perform an anastomosis. Recently, natural orifice specimen extraction (NOSE) and intracorporeal anastomosis have been proposed to minimize abdominal wall trauma and improve the quality of laparoscopic colon resections Objective: To evaluate the feasibility and safe...

Given a 3-axis cutter motion we ask for a geometric representation of the resulting surface. Our discrete solution starts with a regular array of height values initialized e.g. to some constant value. The cutter is positioned at each array element of the scan-converted motion path, and the heights of the elements in the array covered by the cutter are decreased according to the cutter's geometr...

This Article is brought to you for free and open access by the Ocean, Earth & Atmospheric Sciences at ODU Digital Commons. It has been accepted for inclusion in OEAS Faculty Publications by an authorized administrator of ODU Digital Commons. For more information, please contact [email protected]. Repository Citation Anderson, Robert F.; Mawji, Edward; Cutter, Gregory A.; Measures, Christop...

+*Koyama, T; *Sugano, N; *Miki, H; **Murakami, A; *Nishii, T; *Sato, Y; *Yoshikawa, H; *Tamura, S; *Ochi, T +*Osaka University Graduate School of Medicine, Osaka, Japan. [email protected] Introduction It has been reported that almost 100% of hips with extensive type of osteonecrosis of the femoral head (ONFH) show progression of collapse if they do not receive any surgical treatm...

The riotion of “cottipittatiotial crcgtitig ” focuses oti the tiiinierous ~ * a y s in which coniputatiotial niedia tilay be used to expand the expressive range of traditional editcational crufts. One itnportant dimension of this approach involves a close re-examination of titi issue often taketi f o r grtitited iti educational technologytinniely, the desigri and use of I/O devices. The next de...