A label-free optical biosensor based on a one-dimensional photonic crystal microring resonator with enhanced light-matter interaction is demonstrated. More than a 2-fold improvement in volumetric and surface sensing sensitivity is achieved compared to conventional microring sensors. The experimental bulk detection sensitivity is ~248nm/RIU and label-free detection of DNA and proteins is reporte...

With advances in point-of-care testing (POCT), lateral flow assays (LFAs) have been explored for nucleic acid detection. However, biological samples generally contain complex compositions and low amounts of target nucleic acids, and currently require laborious off-chip nucleic acid extraction and amplification processes (e.g., tube-based extraction and polymerase chain reaction (PCR)) prior to ...

We constructed an original supramolecular assembly on a surface of sensor composed of an innovative combination of an engineered cytochrome b5 and a modified nucleic acid bound to a synthetic lipid hemimembrane. The protein/DNA block, called (PDNA) 2, was synthesized and purified before its immobilization onto a hybrid bilayer reconstituted on a gold surface. Surface plasmon resonance (SPR) and...

We propose an ultracompact parallel label-free biosensor based on concentric silicon-on-insulator microring resonators. Our numerical studies show that the proposed biosensor offers higher sensitivity and a larger detection area than conventional single-ring-based sensors. We analyze the detection sensitivity of the DNA hybridization by immobilizing the probe on top of the double concentric rin...

We propose and experimentally verify an innovative label-free optical fiber biosensor based on a Mach-Zehnder interferometer for bovine serum albumin (BSA) concentration detection. The proposed fiber biosensor utilized a micro-cavity within a single-mode fiber to induce Mach-Zehnder interference. A remarkable feature of this biosensor is that external media can directly interact with the fiber ...

The development of a microfluidic biosensor module with fluorescence detection for the identification of pathogenic organisms and viruses is presented in this article. The microfluidic biosensor consists of a network of microchannels fabricated in polydimethylsiloxane (PDMS) substrate. The microchannels are sealed with a glass substrate and packed in a Plexiglas housing to provide connection to...

Monitoring the DNA-Damage Response (DDR) activated pathway in multicellular tumor spheroid models is an important challenge as these 3D models have demonstrated their major relevance in pharmacological evaluation. Herein we present DDR-Act-FP, a fluorescent biosensor that allows detection of DDR activation through monitoring of the p21 promoter p53-dependent activation. We show that cells expre...

Genetically modified organisms (GMOs) are plants or animals whose genetic make-up has been transformed by recombinant DNA technology, which has new features such as resistance to herbicides, virus and insect. Recently, genetic modification of food products has increased in order to reduce world poverty and hunger and increase food production However, the impact of GMOs on the human health is a ...

Piezoelectric materials such as quartz can be used to detect mass change due to their piezoelectric properties. Quartz Crystal Microbalance (QCM) is an extremely sensitive mass sen frequency biosensor mass mea for QCM DNA biosensor was developed. The potential for QCM to be employed as immunosensor sor, capable of measuring sub-nanogram levels by detecting change in the of a quartz crystal reso...