Establishment of an efficient explants surface disinfection protocol is essential for in vitro cell and tissue culture as well as germplasm conservation, such as the case of Grapevine (Vitis spp.) culture. In this research, different procedures for disinfection and regeneration of field-grown grapevine cv. 'Flame seedless' axillary buds were evaluated. The buds were disinfected using either NaO...

Ironwort / Mountain Tea (Sideritis raeseri Boiss & Heldr.) is an endangered (EN) plant species in Albania. This study aimed to develop a rapid clonal propagation protocol using vitro methodologies. The ironwort seeds were pre-treated with three concentrations of GA3 (250, 500, and 1000 mg l-1). During the inoculation stage, two types culture media, Murashige Skoog (MS) Woody Plant Medium (W...

strawberry is a valuable, nutritious, and economically important fruit all over the world including bangladesh. therefore, there is a demand to develop a suitable variety of strawberry. for this purpose, leaf explants from in vitro grown strawberry plantlets were cultured onto ms medium supplemented with different concentrations and combinations of 2,4-d, naa and ba for callus induction. the mo...

Dwarf hygro (Hygrophila polysperma) is an ornamental aquatic plant that changes its leaf colours to pinkish in high light. It is listed as a medicinal plant in medicinal plant lists of Indian states of West Bengal and Karnataka. It is also used as a screening tool for toxicities and a bioindicator to detect and control algae. The study reported in vitro adventitious shoot regeneration from leaf...

Effects of various culture media and growth regulators on micropropagation of cherry rootstock, M × M60 were investigated in the present study. Stem cuttings of the current season growth were taken from mature stockplants and cultured on the establishment medium after disinfection. After four weeks, elongated shoots were cut and subcultured on the proliferation media including MS, DKW, QL and M...

in order to study, in vitro bud induction and shoot regeneration of different explants (shoot tip, axillary bud, hypocotyl and cotyledon) of henbane on ms medium enriched with various concentrations of kinetin (0, 1, 3 and 5 mg.l-1) alone or in combination with indole-3 acetic acid (0, 0.1 and 0.5 mg.l-1) this research was investigated in urom zist tak knowledge-based company plant tissue cultu...

For sexual reproduction of Allium hirtifolium, collected bulbs were planted in the field. In order to develop ovary embryo and seed setting, control self-pollination and natural open pollination took placed using honey bees under cages. Ovary development and seed setting of A. hirtifolium extraordinary were affected by pollination and there was a significant difference between mean effect of se...

Mycoplasma synoviae (MS) is an important avian pathogen that can cause both respiratory disease and joint inflammation synovitis in poultry, inducing economic losses to the Iranian chicken industry especially breeder farms. The aim of this study was to use the MS specific PCR and culture methods in order to detect of M. synoviae from breeder farms where located in Tehran province. A total of 47...

Background & Aim: Clematis is an ornamental and medicinal plant that is primarily planted as an ornamental plant. Clematis orientalis is native to Asia and Central Europe, used as an antiseptic and refrigerant. The propagation of Clematis is very important. In this study, the effects of plant growth regulators on rooting and growth of the plants in vitro cult...