background : glioblastoma multiforme (gbm) or grade iv astrocytoma is the most common and lethal adult malignant brain tumor. several of the molecular alterations detected in gliomas may have diagnostic and/or prognostic implications, proteomics has been widely applied in various areas of science, ranging from the deciphering of molecular pathogen nests of discuses. method: we extracted protein...

alpha-amylase e.c.3.2.1.1. (1,4 glucan, 4 glucanohydrolase) can be obtained from salivary glands, pancreas and microorganisms such as pseudomonas and asperigllus, as well as muscles and ovarian tubes.1.2 alpha-amylase from bacillus subtilis #1024(a tcc 465)* was purified with a highest degree of purity in our laboratory (31.59 u/mg). the extracellular alpha-amylase was subjected to different pu...

multiple myeloma (mm) characterized by proliferation of plasma cells in bone marrow and production of monoclonal immunoglobulin's. recently, arsenic trioxide (ato), has been considered for treatment refractory mm. we assessed the safety and efficacy of ato for patients with refractory mm. a phase 2, study of arsenic trioxide was conducted in 12 mm patients, whose refractory to two standard ther...

a capillary electrophoresis method was used for assay of some degradation products of carvedilol. the optimized parameters were as; running buffer 80 mm acetate dissolved in methanol/ethanol mixture (65:35 v/v), applied voltage of 19 kv, temperature is 20 ºc and the wavelength range of 200-350 nm. the results indicate that the proposed ce method could effectively separate carvedilol from its de...

Abstract The objective of this work was to evaluate microfluidic chip electrophoresis, known as lab-on-a-chip technique, for the detection milk adulteration using cheese whey in comparison with SDS-PAGE. Raw, pasteurized, processed at an ultra-high temperature (UHT), and powdered samples received increasing concentrations (0, 1, 2.5, 5, 10, 20, 30, 50, 100% v/v), were subjected electrophoresis ...

To detect the genome of viruses (in environmental and clinical samples), we use electrophoresis running buffer after PCR reaction. Also, buffers were used widely to separate any DNA molecule. In this paper, four types previously known compare which is easy for preparation, simple in structure, low cost good performance agarose gel electrophoresis. For this, two concentration (1%, 2%) ladder (10...

Two steps of enzymatic oxidations from hypoxanthine to uric acid with xanthine oxidase (XOD) were kinetically analyzed by capillary electrophoresis/dynamic frontal analysis. When a substrate solution was introduced into separation buffer containing XOD, the reaction continuously proceeded during electrophoresis and product resolved zone. A plateau signal detected based on constant rate XOD. The...

Sunscreens are known to protect against sunlight-induced erythema and sunburn, but their efficiency at protecting against skin cancer is still a matter of debate. Specifically, the capacity of sunscreens to prevent or reduce tissue and DNA damage has not been thoroughly investigated. The present study was undertaken to assess the ability of a chemical broad-spectrum sunscreen to protect human s...

Waldenstrom's macroglobulinemia (WM) is lymphoplasmacytoid malignancy that affects B lymphocytes. Cutaneous involvement of WM is rare, but various cutaneous manifestations have been reported. These findings are due to various pathological processes including direct invasion of tumor cells into the skin, deposition of paraproteins, hyperviscosity syndrome, and cryoglobulinemia. A 64-year-old man...

In previous studies, we first isolated one different protein β-1,3-glucanase using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry from normal wheat (Triticum aestivum L.) and chemical hybridization agent-induced male sterility (CIMS) wheat. In this experiment, β-1,3-glucanase activity and the expression of a callose deposition-re...