In vivo aging of human fibroblasts altered proliferative properties but not the potential for novel gene expression in response to muscle trans-acting factors. Heterokaryons produced by fusing fibroblasts with muscle cells permitted a dissociation of the effects of aging on cell division and other cell functions. Skin fibroblasts derived from fetal and adult stages of development were distinct ...

in all engineering applications where solid surfaces slide on each other, friction plays an important role. it becomes even more important, particularly in metal forming processes where the two sliding materials are metallic. among forming processes, forging is an important process, because of improving material structure, mechanical properties and reducing production time. an inverse analysis ...

The control of gene expression during terminal myogenesis was explored in heterokaryons between differentiated and undifferentiated myogenic cells by analyzing the formation of species specific myosin light chains of chick and rat skeletal muscle. Dividing L6 rat myoblasts served as the biochemically undifferentiated parent. The differentiated parental cells were mononucleated muscle cells (myo...

When mouse fibroblasts transformed by SV 40 virus are fused with African Green Monkey kidney cells about 5 % of the heterokaryons produce infective SV 40 virus. If the transformed mouse cells are treated with iododeoxyuridine before fusion, the percentage of heterokaryons producing SV40 virus is increased to between 50 and 80 %; if the transformed cells are treated with 8-azaguanine, the percen...

Heterokaryons derived from polyethylene glycol-mediated fusion of myoblasts at different stages of development were used to investigate the transition of cells in the skeletal muscle lineage from the determined to the differentiated state. Heterokaryons were analyzed by immunofluorescence, using rabbit antibodies against the skeletal muscle isoforms of chicken creatine kinase and myosin, and a ...