A new series of 2-amino-benzo[de]isoquinoline-1,3-diones was synthesized and fully characterized in our previous paper. Here, their cytotoxic effects have been evaluated in vitro in relation to colon HCT-116, hepatocellular Hep-G2 and breast MCF-7 cancer cell lines, using a crystal violet viability assay. The IC50-values of the target compounds are reported in µg/mL, using doxorubicin as a refe...

BACKGROUND Oridonin isolated from Rabdosia rubescens, a plant used to treat cancer in Chinese folk medicine, is one of the most important antitumor active ingredients. Previous studies have shown that oridonin has anti- tumor activities in vivo and in vitro, but little is known about cell cycle effects of oridonin in gastric cancer. MATERIALS AND METHODS MTT assay was adopted to detect the pr...

The cell cycle of antigen-specific T cells in vivo has been examined by using a few methods, all which possess some limitations. Bromodeoxyuridine (BrdU) marks that are or recently completed S-phase, and carboxyfluorescein succinimidyl ester (CFSE) detects daughter after division. However, these dyes do not allow identification the phase at time analysis. An alternative approach is to exploit K...

CKBM is a natural product that exhibits a novel anti-tumor activity through the induction of cell cycle arrest and apoptosis. We have investigated its effects on cell cycle regulation using a gastric cancer cell line, AGS. The effects of CKBM on cell proliferation, cell cycle regulation and apoptosis were analyzed using BrdU (5-bromo-2'-deoxyuridine) cell proliferation assay and flow cytometric...

The mechanism by which the geranylgeranyltransferase I inhibitor GGTI-298 and the farnesyltransferase inhibitor FTI-277 inhibit human tumor growth is not known. Herein, we demonstrate that in the human lung adenocarcinoma A549 cells, GGTI-298 induced a G1-G0 block whereas FTI-277 induced an enrichment in the G2-M phase of the cell cycle. Although FTI-277, GGTI-298, and compactin inhibited A549 ...

in this paper we will prove that the simple group g2(q) where 2 < q = 1(mod3)is recognizable by the set of its order components, also other word we prove that if g is anite group with oc(g) = oc(g2(q)), then g is isomorphic to g2(q).

The research article presents the synthesis, characterization, and biological evaluation of amoxicillin functionalized with AgNPs low toxicity good antimicrobial property which can be produced silver nitrate drug in an environmentally friendly method. synthesized nanoparticles were subjected to characterization by UV-visible spectroscopy, Fourier transform infrared x-ray diffraction, field-emis...

Paclitaxel (taxol) has been used for the treatment of various human tumors and is an exceedingly efficient chemotherapy agent against esophageal cancer. However, the precise molecular mechanisms of paclitaxel effects on human esophageal adenocarcinoma cells are not well understood. MTT assay and cell cycle analysis were performed to examine the mechanism of antiproliferative and cell viability ...

BACKGROUND The present study was designed to determine whether Allium ursinum L (ramson) could inhibit the proliferation of human AGS gastric cancer cells. Furthermore, we attempted to determine whether this inhibition could occur by targeting regulatory elements of the cell cycle. METHODS Flow cytometry was used to observe apoptosis and the cell cycle in AGS cell lines treated or not treated...