An accurate and sensitive reversed-phase high-performance liquid chromatograph-ic method for determination of sertraline in human serum is described using 4-chloro-7-nitrobenzofurazan as pre-column derivatization agent. The drug and an internal standard (azithrimycin) were extracted from serum using a mixture of diethyl ether-chloroform and subjected to the pre-column derivatization with...

29. 3. Syvanen AC. From gels to chips: “minisequencing” primer extension for analysis of point mutations and single nucleotide polymorphisms. Hum Mutat 1999;13:1–10. 4. Xiao W, Oefner PJ. Denaturing high-performance liquid chromatography: a review. Hum Mutat 2001;17:439–74. 5. Oefner PJ. Allelic discrimination by denaturing high-performance liquid chromatography. J Chromatogr B 2000;739:345–55....

Garrido JL and Zapata M (1996) Ion-pair reversed-phase high-performance liquid chromatography of algal chlorophylls. Journal of Chromatography A 738: 285. Hodgson DA, Wright SW and Davies N (1997) Mass spectrometry and reverse phase HPLC techniques for the identiRcation of degraded fossil pigments in lake sediments and their application in palaeolimnology. Journal of Paleolimnology 18: 335. Jef...

complex heat transfer phenomena (including unsteady state conduction, convection and solidification processes) occur in steel belt conveyer sulfur granulating method. numerical simulation of this technique is performed via a comprehensive and multifaceted one dimensional model. since the air situated between the adjacent sulfur pastilles is essentially stagnant, therefore, the surface temperatu...

A comprehensive two-dimensional liquid chromatography system was constructed on the combination of size exclusion chromatography (SEC) and reverse phase liquid chromatography (RPLC). The first dimension used a SEC column with 300 mm x 8mm i.d., packed with Toyopearl HW-40S. The column was eluted with 0.05 mol/l Tris-HCl (pH 6.9) at a flow rate of 0.4 ml/min. The second dimension used a RPLC col...

background and objectives:  high concentration of preservatives in food may result in gastrointestinal disturbances whereby some patients suffering from asthma, rhinitis, or urticaria. the aim of this study is the introduction and optimization a new method for simultaneous determination of four preservatives (sb, ps, mp, pp) in foodstuff by high performance liquid chromatography. materials and ...

Elution and solvation processes in liquid chromatography may be controlled by temperature changes. In the case of solvent adsorption, the temperature influences the amount of adsorbed solvent as well as the enthalpy and entropy of the solvation process. In this work, the thermodynamic parameters of organic solvents used as organic modifiers in the reversed-phase high-performance liquid chromato...