نتایج جستجو برای: hiseq second

تعداد نتایج: 616088  

Journal: :Molecular and Cellular Endocrinology 2016
Michal Swierniak Aleksandra Pfeifer Tomasz Stokowy Dagmara Rusinek Mykola Chekan Dariusz Lange Jolanta Krajewska Małgorzata Oczko-Wojciechowska Agnieszka Czarniecka Michal Jarzab Barbara Jarzab Bartosz Wojtas

The molecular etiology of follicular thyroid tumors is largely unknown, rendering the diagnostics of these tumors challenging. The somatic alterations present in these tumors apart from RAS gene mutations and PAX8/PPARG translocations are not well described. To evaluate the profile of somatic alteration in follicular thyroid tumors, a total of 82 thyroid tissue samples derived from 48 patients ...

2016
Jigna D. Shah Prerak T. Desai Ying Zhang Sarah K. Scharber Joshua Baller Zheng S. Xing Carol J. Cardona Tjeerd Kimman

Several RNA viruses such as astrovirus, rotavirus, reovirus and parvovirus have been detected in both healthy and diseased commercial poultry flocks. The aim of this study was to characterize (a) the development of the RNA viral community in the small intestines of healthy broiler chickens from hatch through 6 weeks of age (market age) and (b) the contribution of the breeder source vs. bird age...

2017
Jinny A. Paul Anupam Aich Juan E. Abrahante Ying Wang Rebecca S. LaRue Susan K. Rathe Krystina Kalland Aditya Mittal Ritu Jha Fei Peng David A. Largaespada Anindya Bagchi Kalpna Gupta

Pain is a hallmark feature of sickle cell disease (SCD). Recurrent and unpredictable acute pain due to vaso-oclussive crises (VOC) is unique to SCD; and can be superimposed on chronic pain. To examine the mechanisms underlying pain in SCD, we performed RNA sequencing of dorsal root ganglion (DRG) of transgenic sickle mice and their age-matched control mice expressing normal human hemoglobin A, ...

2014
Amrit Singh Casey P Shannon Gail M Gauvreau Paul M O'Byrne J Mark FitzGerald Louis-Philippe Boulet Scott J Tebbutt

Methods 35 individuals (17 ERs and 18 DRs) participated in the allergen inhalation challenge. Blood samples were obtained prior to and 2 hours post allergen inhalation challenge. HiSeq-Illumina paired-ends 100bp sequencing was performed. A UCSC transcriptome using both the UCSC gene and gene-isoform transcripts was created using the RSEM (RNA-Seq by Expectation Maximization) package. RSEM uses ...

2015
Zhihua Jiang

During the last ten years, next generation sequencing methods, technologies and platforms have revolutionized genomics and transcriptomics research fields and advanced their applications in agriculture and biomedicine [1–3]. To date, the Roche 454 GS FLX(+) system, Applied Biosystems SOLiD (supported oligonucleotide ligation and detection) and Ion Proton/PGM/Chef systems now owned by Life Techn...

2015
Carol A. Wilson CAROL A. WILSON

Iris gatesii is a rare bearded species in subgenus Iris section Oncocyclus that occurs in steppe communities of southeastern Turkey. This species is not commonly cultivated, but related species in section Iris are economically important horticultural plants. The complete plastid genome is reported for I. gatesii based on data generated using the Illumina HiSeq platform and is compared to genome...

2017
Zheng Zhao Fanlin Meng Wen Wang Zheng Wang Chuanbao Zhang Tao Jiang

Gliomas are the most common and lethal intracranial tumours. RNA sequencing technologies and advanced data analyses recently enabled the characterization of transcriptomic information, including protein-coding gene expression, non-coding gene expression, alternative splicing, and fusion gene detection, to facilitate detection of diseases and altered phenotypes. As a part of the Chinese Glioma G...

2016
Simon T. Maddock Andrew G. Briscoe Mark Wilkinson Andrea Waeschenbach Diego San Mauro Julia J. Day D. Tim J. Littlewood Peter G. Foster Ronald A. Nussbaum David J. Gower

Mitochondrial genome (mitogenome) sequences are being generated with increasing speed due to the advances of next-generation sequencing (NGS) technology and associated analytical tools. However, detailed comparisons to explore the utility of alternative NGS approaches applied to the same taxa have not been undertaken. We compared a 'traditional' Sanger sequencing method with two NGS approaches ...

2017
Jianhua Guo Bing-Jie Ni Xiaoyu Han Xueming Chen Philip Bond Yongzhen Peng Zhiguo Yuan

The data in this article mainly present the sequences of activated sludge from a full-scale municipal wastewater treatment plant (WWTP) carrying out simultaneous nitrogen and phosphorous removal in Beijing, China. Data include the operational conditions and performance, dominant microbes and taxonomic analysis in this WWTP, and function annotation results based on SEED, Clusters of Orthologous ...

Journal: :Methods in molecular biology 2017
Blanca Majem Feng Li Jie Sun David T W Wong

Salivary biomarkers for disease detection, diagnostic and prognostic assessments have become increasingly well established in recent years. In this chapter we explain the current leading technology that has been used to characterize salivary non-coding RNAs (ncRNAs) from the extracellular RNA (exRNA) fraction: HiSeq from Illumina® platform for RNA sequencing. Therefore, the chapter is divided i...

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