نتایج جستجو برای: hiseq second
تعداد نتایج: 616088 فیلتر نتایج به سال:
paris polyphylla smith var. yunnanensis (liliaceae) is an important traditional medicinal plant of the yunnan province in china. however, the genomic information regarding this plant is limited. to further understand its molecular background, we conducted illumina hiseq 2000 second-generation sequencing of this plant species. approximately 30,198,679 reads with an average length of 202 bases we...
DNA assembly is a core methodological step in metagenomic pipelines used to study the structure and function within microbial communities. Here we investigate the utility of Pacific Biosciences long and high accuracy circular consensus sequencing (CCS) reads for metagenomic projects. We compared the application and performance of both PacBio CCS and Illumina HiSeq data with assembly and taxonom...
BACKGROUND Identification of gene variants plays an important role in research on and diagnosis of genetic diseases. A combination of enrichment of targeted genes and next-generation sequencing (targeted DNA-HiSeq) results in both high efficiency and low cost for targeted sequencing of genes of interest. METHODOLOGY/PRINCIPAL FINDINGS To identify mutations associated with genetic diseases, we...
Background More extensive use of metagenomic shotgun sequencing in microbiome research relies on the development of high-throughput, cost-effective sequencing. Here we present a comprehensive evaluation of the performance of the new high-throughput sequencing platform BGISEQ-500 for metagenomic shotgun sequencing and compare its performance with that of 2 Illumina platforms. Findings Using fe...
Germline and somatic variant identification using BGISEQ-500 and HiSeq X Ten whole genome sequencing
Technological innovation and increased affordability have contributed to the widespread adoption of genome sequencing technologies in biomedical research. In particular large cancer research consortia have embraced next generation sequencing, and have used the technology to define the somatic mutation landscape of multiple cancer types. These studies have primarily utilised the Illumina HiSeq p...
The complete genome sequence of Lactobacillus crustorum deciphered by PacBio RS II and Illumina HiSeq 4000 sequencing was first reported with one chromosome and two plasmids. Sequence analysis of L. crustorum MN047 showed probiotic characteristics.
Multiplexing is a widely-used procedure that allows multiple DNA libraries to be pooled together for efficient sequencing. However, recent reports suggest that the DNA barcodes that label different libraries can “swap” on patterned flow-cell Illumina sequencing machines, including the HiSeq 4000, HiSeq X, and NovaSeq, thereby mislabelling molecules [1, 2]. This may compromise many types of -omi...
Bacillus solimangrovi GH 2-4T was isolated from mangrove soil and subjected to whole genome sequencing on HiSeq platform and annotated on RAST. The nucleotide sequence of this genome was deposited into DDBJ/EMBL/GenBank under the accession MJEH00000000.
Using Illumina HiSeq and PacBio technologies, we sequenced the genome of the multidrug-resistant bacterium Staphylococcus haemolyticus, originating from a bloodstream infection in a neonate. The sequence data can be used as an accurate reference sequence.
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