Different immunoassay-based methods have been devised to detect protein targets. These methods have some challenges that make them inefficient for assaying ultra-low-amounted proteins. ELISA, iPCR, iRCA, and iNASBA are the common immunoassay-based methods of protein detection, each of which has specific and common technical challenges making it necessary to introduce a novel method in order to ...

An ultrasensitive microRNA assay was developed with one-step loop-mediated isothermal amplification (LAMP) initiated by the target microRNA.

The methods of thermogravimetric analysis (TGA) and differential scanning calorimettry (DSC) have been used to study the thermal decomposition of ammonium perchlorate (AP). TGA curves obtained under both isothermal and nonisothermal conditions show a characteristic slowdown at the extents of conversion, R ) 0.30-0.35. DSC demonstrates that in this region the process changes from an exothermic t...

Isothermal DNA amplification is an alternative to PCR-based amplification for point-of-care diagnosis. Since the early 1990s, the approach has been refined into a simple, rapid and cost-effective tool by means of several distinct strategies. Input signals have been diversified from DNA to RNA, protein or small organic molecules by translating these signals into input DNA before amplification, t...

In this article, failure and fracture mechanisms in an aluminum alloy (which has been used in diesel internal combustion engines), with and without ceramic thermal barrier coatings, have been investigated under isothermal and non-isothermal fatigue loadings. In this research, the base material is an aluminum-silicon-magnesium alloy and the thermal barrier coating includes a metallic bond coat l...

This paper presents the dynamic neural networks partial least squares (DNNPLS) as a strategy for open-loop identification of multivariable chemical processes that circumvent some of the difficulties associated with multivariable process control. The DNNPLS is an extension of the neural networks’ partial least squares (NNPLS) developed by Qin and McAvoy (Comp. Chem. Eng. 20 (1992) 379). Here, a ...

As part of a systematic study devoted to the diagnostic of solar-like coronal structures in late-type stars, we have analyzed ROSAT/PSPC X-ray spectra of eight main sequence G-type stars in the solar neighborhood. We have fitted the X-ray spectra adopting two different classes of models: the usual two-component isothermal models and the more physically meaningful hydrostatic loop models. The tw...

Volume penalization is a subclass of immersed boundary methods for modeling complex geometry flows, which introduces the effects of obstacles by modifying the governing equations. The method presented in this paper encompasses general boundary conditions as an extension of the Brinkman Penalization Method (BPM) [1], which was originally developed for solid, isothermal obstacles in incompressibl...

PG9 is the founder member of an expanding family of glycan-dependent human antibodies that preferentially bind the HIV (HIV-1) envelope (Env) glycoprotein (gp) trimer and broadly neutralize the virus. Here, we show that a soluble SOSIP.664 gp140 trimer constructed from the Clade A BG505 sequence binds PG9 with high affinity (∼11 nM), enabling structural and biophysical characterizations of the ...