Nano-composite copper-zinc oxide (NCCZO) was deposited on borosilicate glass using a novel sol-gel/doctor-blade technique. The photocatalyst was prepared from copper acetylacetonate, zinc acetylacetonate, monoethanolamine (MEA) and citric acid monohydrate at 75 oC, aged for 1 d and annealed at 650 oC. The properties of the NCCZO nano-composite were investigated by X-ray diffraction (XRD) and fi...

The kinetics of the reactions between triphenylphosphine 1 and dialkyl acetylenedicarboxylates 2, in the presence of a NH-acid such as 5-nitroindazole 3,were studied. Corresponding kinetic parameters to all reactions were evaluated, with the second order rate constant (k) values calculated. Effects of solvent, temperature, and reactant...

We have characterized the binding of rhodamine phalloidin to actin filaments and actin filaments saturated with either myosin subfragment-1 or tropomyosin in 50 mM KCl, 1 mM MgCl2 buffer at pH 7.0. Direct transient kinetic measurements of rhodamine phalloidin binding to actin filaments indicate an association rate constant of 2.8 x 10(4) M-1 s-1 and a dissociation rate constant of 4.8 x 10(-4) ...

Mass spectrometry (MS)-based techniques have enormous potential for kinetic studies on enzyme-catalyzed processes. In particular, the use of electrospray ionization (ESI) MS for steady-state measurements is well established. However, there are very few reports of MS-based studies in the pre-steady-state regime, because it is difficult to achieve the time resolution required for this type of exp...

Extreme instability of pyrimidine motif triplex DNA at physiological pH severely limits its use for artificial control of gene expression in vivo. Stabilization of the pyrimidine motif triplex at physiological pH is therefore of great importance in improving its therapeutic potential. To this end, isothermal titration calorimetry interaction analysis system and electrophoretic mobility shift as...

3 H-Spiroperidol, a butyrophenone antipsychotic agent, binds specifically and with high affinity to neuroleptic receptors in the 3 corpus striatum and substantia nigra. Scatchard analysis of Hspiroperidol isotherms in the rat striatum revealed a dissociation constant (KQ) of 30 pM and a total receptor density (Bmax) of 430 fmoles/mg protein. In the substantia nigra, the and Bmax values were 42 ...

A mathematical model is developed to describe the contaminant transport, sorption, and biodegradation in saturated porous media. In the model development, sorption was accounted for as a kinetic process for the contaminant transport. In addition, a double Monod function was incorporated into the model to describe the biodegradation of contaminants and utilization of oxygen. In the description o...

We have studied the ATP-induced allosteric structural transition of GroEL using small angle X-ray scattering and fluorescence spectroscopy in combination with a stopped-flow technique. With X-ray scattering one can clearly distinguish the three allosteric states of GroEL, and the kinetics of the transition of GroEL induced by 85 microM ATP have been observed directly by stopped-flow X-ray scatt...

A reaction system of Michaelis-Menten enzyme on single substrate can be characterized by the initial substrate concentration before enzyme action (S0), the maximal reaction rate (Vm) and Michaelis-Menten constant (Km), besides some other required parameters. The estimations of S0, Vm and Km can be used to measure enzyme substrates, enzyme activities, epitope or hapten (enzyme-immunoassay), irre...

Formulations are presented that describe the concentration dependency of nutrient-limited transport and growth in molecular terms. They relate the rate of transport at steady state through a two-sequence process, transport and metabolism, to ambient concentrations according to the amounts and kinetic characteristics of the two rate-limiting proteins in these sequences. Sequences are separated b...