Photoelectro-Fenton (PEF) process can continuously promote the occurrence of Fenton reaction and generation active species, which is an advanced oxidation technology for pollutant degradation. However, lack bifunctional catalysts restricts development PEF technology. In this study, electronic rearrangement MOF-525 modified by metalloporphyrin (named MOF-525-Fe/Zr) was prepared, to load on carbo...

The modification of silica gel, from the waste bricks burning process, with diphenylcarbazone has been done. gel modified by (Si-DPZon) was obtained boiling ash NaOH solution to create sodium silicate and neutralized HCl get then physically ligand. study included determination adsorbent functional groups spectrophotometer IR, surface acidity acid-base titration method, specific area absorption ...

In-cell NMR is a unique approach to observe the structural and dynamic properties of biological macromolecules at atomic resolution directly in living cells. Protein folding, chemical modifications, conformational changes induced by ligand binding can be observed. Therefore, this method has great potential context drug development. However, short lifetime human cells confined spectrometer limit...

We extended characterization of mutational substitutions in the ligand-binding region of Trg, a low-abundance chemoreceptor of Escherichia coli. Previous investigations using patterns of adaptational methylation in vivo led to the suggestion that one class of substitutions made the receptor insensitive, reducing ligand-induced signaling, and another mimicked ligand occupancy, inducing signaling...

In our previous studies, we demonstrated that the mutation of His393(6.55) to alanine results in an increased affinity of 1,4-disubstituted phenylpiperazines to the dopamine D(2L) receptor. This change most likely accounts for the reduced steric hindrance in this part of the binding pocket. In this work, we investigated the role of the steric hindrance imposed by the residue His393(6.55) for th...

The accurate calculation of the free energy of interaction of protein-water-ligand systems has an important role in molecular recognition and drug design that is often not fully considered. We report free energy thermodynamic integration calculations used to evaluate the effects of inclusion, neglect, and targeting and removal (i.e., systematic substitution by ligand functional groups) of an im...

GC-C (guanylate cyclase C) is the receptor for heat-stable enterotoxins, guanylin and uroguanylin peptides. Ligand binding to the extracellular domain of GC-C activates the guanylate cyclase domain leading to accumulation of cGMP. GC-C is expressed as differentially glycosylated forms in HEK-293 cells (human embryonic kidney-293 cells). In the present study, we show that the 145 kDa form of GC-...

The mercury (II) complex, Hg (Meca2en)I2 (1), containing bidentate Schiff base ligand  Meca2en = N,N′-bis[(E)-2-benzylidenepropylidene]ethane-1,2-diamine, has been synthesized and characterized by elemental analyses (CHN) and 1H-NMR spectroscopy. The crystal structure of 1 was determined using single-crystal X-ray diffraction. Structural investigation shows that 1 crystallizes in a monoclinic s...

or partially covalent, bond of the protein under study (Cohen, 1970), hence also the more general term 'covalent modification' (Brocklehurst, 1982). Protein modification studies have so far aimed at: (a) the determination of the kind and number of reactive groups of the protein molecule under study, (b) the development of site-specific protein modifying agents, and (c) the elucidation of enzyme...