A coumarin-fused diazirine photolabeling agent exhibited dramatic enhancement in fluorescence after cross-linking with the target protein. Fluorescence emission from the coumarin moiety was efficiently quenched by the diazirine group, and was then intensively recovered from photolysis treatment by irradiation with light at a wavelength of 365 nm.

This paper examines the use of genetic programming and agent-based modeling as an isomorphic analog of bounded rationality in individual decision making. This approach is explored in the context of land change modeling in the southern Yucatán peninsular region of Mexico. Results are reported for experiments linking varied implementations of genetic programming to features of bounded rationality.

purpose: to evaluate the efficacy of riboflavin administration and ultraviolet a (uva) cross-linking on advanced symptomatic bullous keratopathy (bk). methods: 10 patients with symptomatic pseudophakic bullous keratopathy (pbk) were included. slit-lamp examination, visual acuity, foreign body sensation (fbs) and corneal thickness as measured by pentacam, were performed before corneal cross-link...

patients with keratoconus constitute a large proportion of patients suffering from anterior segment disorders. as of this time, however, its pathogenesis has not yet been elucidated. a reduction in cross-link between collagen molecules has been observed in keratoconic eyes as compared to normal ones. present approaches only address refractive errors resulting from the abnormal abnormal corneal ...

A novel layered graphene-based architecture is achieved via an ordered self-assembly process. Amphipathic graphene nanosheets are joined horizontally into large sheets via edge splicing, and a cross-linking agent of poly(vinyl alcohol) bridges them into integrated three-dimensional monoliths with tunable interlayer spacing. This layered architecture possesses highly ordered and favorable microc...

Biocompatible and robust gold nanoparticles (AuNPs) were prepared via a simple synthetic strategy employing dextran as a reducing agent and surface coating material. Cross-linking and amination of the surface-coated dextran significantly improved the colloidal stability of AuNPs and rendered the nanoparticles more amenable to bioconjugation.

Mycoplasma species bovine group 7 bound plasminogen at the cell surface in a lysine-dependent manner. Cell-bound plasminogen was rapidly activated to plasmin by exogenous urokinase, and this activity was associated with plasminogen binding capacity. Binding assays using plasminogen modified with a trifunctional cross-linking agent revealed several binding proteins.

This paper presents the successful preparation of porous membranes based on chitosan with enhanced mechanical, thermal and chemical properties applicable in water treatment field. Herein, chitosan/kaolin composite a cross-linking agent porogen were prepared using solvent casting method. The characterization as-fabricated indicated that combined effect kaolin as reinforcing agent, polyethylene g...