This paper discusses recent research on methods for estimating configuration parameters for the Matrix Comparator used for linking unstandardized or heterogeneously standardized references. The matrix comparator computes the aggregate similarity between the tokens (words) in a pair of references. The two most critical parameters for the matrix comparator for obtaining the best linking results a...

In experiments on isolated human erythrocyte membranes, cross-links between protein components have been produced both by the catalyzed oxidation of intrinsic sulfhydryl groups with the o-phenanthroline cupric ion complex and by reaction with dimethyl dithiobispropionimidate, a bisalkylimidate containing a disulfide linkage between the two reactive groups. The mixture of molecular species has b...

CTP:phosphocholine cytidylyltransferase (CCT), a key enzyme that controls phosphatidylcholine synthesis, is regulated by reversible interactions with membranes containing anionic lipids. Previous work demonstrated that CCT is a homodimer. In this work we show that the structure of the dimer interface is altered upon encountering membranes that activate CCT. Chemical cross-linking reactions were...

Hydrogels, highly hydrated cross-linked polymer networks, have emerged as powerful synthetic analogues of extracellular matrices for basic cell studies as well as promising biomaterials for regenerative medicine applications.[1] A critical advantage of these artificial matrices over natural networks is that bioactive functionalities, such as cell adhesive sequences and growth factors, can be in...

The method described here allows the mapping of protein-DNA interactions through the ability of formaldehyde to cross-link proteins and nucleic acids in living cells. Formaldehyde is a very reactive dipolar compound which reacts with the amino groups of proteins and amino acids (1, 2). It shows no reactivity however towards free double-stranded DNA, and thus does not cause the extensive DNA dam...