Aim 3,4-methylenedioxymethamphetamine (MDMA) is one of the most widespread illegal drugs, used particularly by young people in the 15-34 age group. MicroRNAs (miRNAs) are endogenously synthesized, non-coding and small RNAs that post-transcriptionally regulate their target genes’ expression by inhibiting protein translation or degradation. miRNAs are increasingly implicated in drug-related...

BACKGROUND Given the emerging roles of miRNAs as potential posttranscriptional/posttranslational regulators of the steroidogenic process in adrenocortical and gonadal cells, we sought to determine miRNA profiles in rat adrenals from animals treated with vehicle, ACTH, 17α-E2 or dexamethasone. Key observations were also confirmed using hormone (Bt2cAMP)-treated mouse Leydig tumor cells, MLTC-1, ...

micrornas (mirnas) are short rna molecules which bind to target mrnas, resulting in translational repression and gene silencing and are found in all eukaryotic cells. approximately 2200 mirna genes have been reported to exist in the mammalian genome, from which over 1000 belong to the human genome. many major cellular functions such as development, differentiation, growth, and metabolism are kn...

Synonymous codons are widely selected for various biological mechanisms in both prokaryotes and eukaryotes. Recent evidence suggests that microRNA (miRNA) function may affect synonymous codon choices near miRNA target sites. To better understand this, we perform genome-wide analysis on synonymous codon usage around miRNA target sites in four plant genomes. We observed a general trend of increas...

Decoding the patterns of miRNA regulation in diseases are important to properly realize its potential in diagnostic, prog- nostic, and therapeutic applications. Only a handful of studies computationally predict possible miRNA-miRNA interactions; hence, such interactions require a thorough investigation to understand their role in disease progression. In this paper, we design a novel computation...

objective(s): vascular calcification is one the major characteristics in patients with various types of chronic inflammatory disorders. mirnas have been shown to be involved in many normal biological functions as well as diseases; however, their role in vascular calcification has not received much attention. materials and methods: in the current study, we built a vascular calcification rat mode...

micrornas (mirnas) are short, endogenous non-coding rnas that function as guide molecules to regulate transcription of their target messenger rnas. several methods including low-density qpcr arrays are being increasingly used to profile the expression of these molecules in a variety of different biological conditions. reliable analysis of expression profiles demands removal of technical variati...